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Production of Validamycins from Crude Substrates by Streptomyces hygroscopicus in an
External-loop Airlift Bioreactor with a Low Height-to-Diameter Ratio

ZHENG Yuguo; CHEN Xiaolong; WANG Zhao; SHEN Yinchu   

  1. Institute of Bioengineering, Zhejiang University of Technology, Hangzhou 310014, China
  • Received:1900-01-01 Revised:1900-01-01 Online:2004-02-28 Published:2004-02-28
  • Contact: ZHENG Yuguo

低高径比外循环气升式生物反应器带渣发酵生产有效霉素

郑裕国; 陈小龙; 汪钊; 沈寅初   

  1. Institute of Bioengineering, Zhejiang University of Technology, Hangzhou 310014, China
  • 通讯作者: 郑裕国

Abstract: Fermentation experiments to produce validamycins from crude substrates by Streptomyces
hygroscopicus were carried out in an external-loop airlift bioreactor (0.0115 m3 ) with a
low ratio of height to diameter of the riser of 2.9 and a ratio of riser to downcomer
diameter of 6.6. The influences of gas flow rate and liquid volume on fermentation of
validamycins were investigated. Comparisons of validamycin fermentation were made among the
external-loop airlift bioreactor, a mechanically stirred tank bioreactor (0.010m3 ) and
shaking flasks. Under the same operation conditions including fermentation medium
composition, inoculum ratio and culture temperature,the fermentation time in the external-
loop airlift bioreactor (45 h) was shorter than that in the shaking flasks (100 h)and the
same as that in the mechanically stirred tank bioreactor. After a total fermentation time
of 45 h under optimized operation conditions, average validamycin concentration obtained in
the external-loop airlift bioreactor was close to 19630μg.m1-1 validamycin concentration
in the mechanically stirred tank bioreactor. It was demonstrated that the external-loop
airlift bioreactor could substitute for the mechanically stirred tank bioreactor in
production of validamycins from crude substrates with dregs by Streptomyces hygroscopicus.

Key words: airlift bioreactor, validamycins, Streptomyces hygroscopicus

摘要: Fermentation experiments to produce validamycins from crude substrates by Streptomyces
hygroscopicus were carried out in an external-loop airlift bioreactor (0.0115 m3 ) with a
low ratio of height to diameter of the riser of 2.9 and a ratio of riser to downcomer
diameter of 6.6. The influences of gas flow rate and liquid volume on fermentation of
validamycins were investigated. Comparisons of validamycin fermentation were made among the
external-loop airlift bioreactor, a mechanically stirred tank bioreactor (0.010m3 ) and
shaking flasks. Under the same operation conditions including fermentation medium
composition, inoculum ratio and culture temperature,the fermentation time in the external-
loop airlift bioreactor (45 h) was shorter than that in the shaking flasks (100 h)and the
same as that in the mechanically stirred tank bioreactor. After a total fermentation time
of 45 h under optimized operation conditions, average validamycin concentration obtained in
the external-loop airlift bioreactor was close to 19630μg.m1-1 validamycin concentration
in the mechanically stirred tank bioreactor. It was demonstrated that the external-loop
airlift bioreactor could substitute for the mechanically stirred tank bioreactor in
production of validamycins from crude substrates with dregs by Streptomyces hygroscopicus.

关键词: airlift bioreactor;validamycins;Streptomyces hygroscopicus