Abstract: The biomass pulse-response method was established and the biomass transmission index was introduced as a quantitative parameter for evaluating the biomass-adsorbent interactions in the expanded bed.With baker’s yeast intact cells and cell debris as the model biomass and anion exchanger Streamline DEAE as the model adsorbent, a series of factors were investigated to optimize the evaluation method.The appropriate operation conditions were chosen as the OD₆₀₀ of biomass pulse at the range of 0.5—0.6, and pulse loading at 80% volume of sedimented bed, expansion factor at 2.5.The method was then used to study the influence of ionic strength of fluid phase on the biomass-adsorbent interactions.The consistent results and slight measurement errors demonstrated that the biomass pulse-response method established in the present work is feasible, reliable and effective for quantitative evaluation of biomass-adsorbent interactions in the expanded bed.

Key words: 扩张床吸附, 脉冲响应法, 生物质固体颗粒, 穿透指数

关键词: 扩张床吸附, 脉冲响应法, 生物质固体颗粒, 穿透指数