CIESC Journal ›› 2004, Vol. 55 ›› Issue (3): 497-500.
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GAN Yiru;HUANG Yongdong;WU Lei
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Published:
甘一如; 黄永东; 吴蕾
Abstract: By using ion-exchange preparative chromatography (IEPC) and reversed-phase high performance liquid preparative chromatography(RP-HPLPC), thymosin α1 was isolated and purified from the crude product synthesized by the solid-phase peptide synthesis(SPPS) method. The purity of the final product reached 95% through ion-exchange chromatography on DEAE Sepharose Fast Flow chromatography and Delta-PakTM C18 purification after optimizing the chromatographic conditions. The capacity of purification process was 50mg/circle. The total yield was 36%. The technology is simple and reliable, and can be scaled up easily.
Key words: 胸腺素α1, 固相多肽合成, 离子交换色谱, 反相高效液相色谱, 分离, 纯化
关键词: 胸腺素α1, 固相多肽合成, 离子交换色谱, 反相高效液相色谱, 分离, 纯化
GAN Yiru, HUANG Yongdong, WU Lei. APPLICATION OF LIQUID CHROMATOGRAPHY FOR ISOLATION AND PURIFICATION OF SOLID-PHASE SYNTHETIC THYMOSIN α1[J]. CIESC Journal, 2004, 55(3): 497-500.
甘一如, 黄永东, 吴蕾. 应用液相色谱法分离纯化固相化学合成胸腺素α1 [J]. 化工学报, 2004, 55(3): 497-500.
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