Abstract: Expanded bed adsorption (EBA) has been widely used in the industrial downstream bioprocessing. Solid matrix is the principal pillar supporting the successful application of EBA. A novel spherical cellulose-TiO2 composite matrix was prepared with the method of water-in-oil suspension thermal regeneration. After activating by epichlorohydrin and coupling with diethylamine,the matrix was obtained to function as an anion exchanger. The experiments in expanded bed indicated that the adsorbent exhibited a stable expansion,approximate plug flow and the comparable column efficiency with the case in packed bed, providing a bovine serum albumin(BSA) adsorption capacity of 42.6 mg•ml^-1 adsorbent at 10% breakthrough.

Key words: 扩张床吸附, 纤维素, 钛白粉, 离子交换, 蛋白质

摘要： 采用反相悬浮热再生法，将超细钛白粉颗粒包埋于纤维素骨架之中，经环氧氯丙烷活化后与二乙胺反应，制得球形阴离子交换吸附剂.研究表明: 吸附剂的扩张行为遵从Richardson-Zaki准则，扩张床内的流动接近于平推流；以牛血清白蛋白(BSA)为模型蛋白质，发现其在扩张床中的穿透行为与固定床中接近，10％穿透时的吸附容量为42.6 mg BSA•ml^-1.

关键词: 扩张床吸附, 纤维素, 钛白粉, 离子交换, 蛋白质