Abstract: Kinetic model of conversion process for the production of D-N-carbamoyl phenylalanine from benzylhydantoin by hydantoinase purified from Burkholderia cepecia njut1 was developed. The study indicated important impact of substrate racemization on enzymatic conversion. The influence of temperature on bioconversion was discussed. The values of solubility and solution constant, racemization constant and Michaelis constant were determined at different temperatures. Temperature was important in conversion of benzylhydantoin and increasing thermostability of hydantoinase was significant for production of D-amino acids.

Key words:

D-海因酶, 苄基海因, 动力学, 温度

摘要： 利用从Burkholderia cepecia njut1中纯化得到的纯海因酶建立了利用苄基海因生产N-氨甲酰-D-苯丙氨酸的酶转化过程的动力学模型.研究表明，底物的消旋对转化有重要影响.探讨了温度对转化过程的影响，测定了不同温度下苄基海因的溶解常数、消旋常数及米氏常数.结果表明，温度对苄基海因转化过程有重要影响，提高海因酶的热稳定性对D-氨基酸的生产有重要意义.

关键词:

D-海因酶, 苄基海因, 动力学, 温度