Abstract: The experimental conditions, including temperature, pH, denatured protein concentration and urea concentration, in the renaturation process of recombinant human interferon-γ inclusion body by dilution were studied and at the same time pulse renaturation was tested. The optimum renaturation conditions were obtained. Results showed that urea could suppress the aggregation of protein during the renaturation process and the suitable urea concentration increased with the increase of denatured protein concentration. The activity and specific bioactivity was up to 2.39×10⁵ IU•ml^-1 and 2.21×10⁷ IU•mg^-1 respectively under 4 ℃, pH 8.0, 1.0 mol•L^-1 urea, 0.1 mg•ml^-1 protein and pulse renaturation operation mode.

Key words: 重组人γ-干扰素, 包涵体, 稀释复性

摘要： 采用稀释法研究了重组人γ-干扰素包涵体复性条件，如温度、pH值、蛋白浓度、复性液中脲浓度，同时对加样操作方式进行了考察，得到了较为适宜的复性条件.结果表明，脲能有效地抑制复性过程中蛋白质的聚集，合适的脲浓度随复性蛋白浓度的增加而有所增大，在4℃、pH值8.0、脲浓度1.0mol•L^-1、蛋白浓度0.1mg•ml^-1及脉冲加样操作方式等条件下，复性后重组人γ-干扰素的活性为2.39×10⁵ IU•ml^-1，比活达2.21×10⁷ IU•mg^-1.

关键词: 重组人γ-干扰素, 包涵体, 稀释复性