Abstract: A bioreactor system with a total working volume of 4.8 L，composed of four airlift suspended-bed bioreactors, one of which was designated for seed cultivation and the others in series for ethanol fermentation, was established and continuously operated for 40 days. A self-flocculating fusant from Saccharomyces cerevisiae and Schizosaccharomyces pombe (SPSC01) was used and ethanol and yeast were co-produced from corn dry milling, two-stage enzymatic hydrolysis (raw material residues were separated after saccharification).The results showed that average levels of 98 g•L^-1 for ethanol, 4 g•L-1 for residual reduced sugar and 6—9 g•L^-1 for residual total sugar were achieved when the medium containing total sugar of 220 g•L^-1 was fed at a dilution rate of 0.05 h-1.Pure yeast, equivalent to 5% (mass) of ethanol and containing protein as high as 48.6%(mass), was co-produced. Ethanol production is expected to be more economically competitive as this high quality yeast potentially can be further processed as value-added by-products.

Key words: 自絮凝颗粒酵母, 酒精发酵并联产酵母, 悬浮床生物反应器

关键词: 自絮凝颗粒酵母, 酒精发酵并联产酵母, 悬浮床生物反应器