CIESC Journal ›› 2004, Vol. 55 ›› Issue (7): 1033-1040.
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LI Ming;JanChrister Janson;SU Zhiguo
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李明;JanChrister Janson; 苏志国
Abstract: The impressing from immobilized medium and moving solution might cause protein conformational change and accompanying loss of protein activity during separation by chromatographic processes. At the same time, the denatured protein might refold to gain their native structure and total activity when they pass through the chromatographic column at suitable operation condition. This review focuses on recent advances in column separation and refolding, which have shown promising results on activity recovery from native protein and refolding yield from inclusion bodies. Suitable chromatographic medium and optimized operation system will affect conformational change and improve the yield of active proteins, which are valuable on both laboratory research and commercial productivity.
Key words: 层析, 蛋白质, 变性, 复性
摘要: 在层析分离过程中,层析的固相介质和溶液环境会引起蛋白质的结构变化:天然的蛋白质可能会降低活性甚至完全失去活性,而结构变化了的蛋白质也可能重新折叠恢复活性.此类现象已经引起研究者特别是生化工程学家的关注,层析介质的种类和操作手段是影响此两个过程的关键因素.利用不同的层析方法,对不同的蛋白质采取相应的措施有利于提高活性蛋白质的回收率,具有重要的理论和应用价值.
关键词: 层析, 蛋白质, 变性, 复性
LI Ming, JanChrister Janson, SU Zhiguo. PROTEIN DENATURATION AND REFOLDING DURING CHROMATOGRAPHIC PROCESSES[J]. CIESC Journal, 2004, 55(7): 1033-1040.
李明, JanChrister Janson, 苏志国. 蛋白质在层析过程中的失活与复性 [J]. 化工学报, 2004, 55(7): 1033-1040.
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