IMMOBILIZED CULTURE OF Bacillus subtilis IN SA/CS-CaCl2/PMCG MICROCAPSULE FOR PRODUCTION OF NATTOKINASE

CIESC Journal ›› 2004, Vol. 55 ›› Issue (8): 1319-1323.

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IMMOBILIZED CULTURE OF Bacillus subtilis IN SA/CS-CaCl₂/PMCG MICROCAPSULE FOR PRODUCTION OF NATTOKINASE

MEI Lehe;ZHANG Xiaozhong;AI Biying;SHENG Qing;LIN Dongqiang;YAO Shanjing;ZHU Ziqiang

Online:2004-08-25 Published:2004-08-25

SA/NaCS-CaCl₂/PMCG微胶囊固定化枯草杆菌HL-1发酵生产纳豆激酶

梅乐和;章小忠;艾碧英;盛清;林东强;姚善泾;朱自强

浙江大学化学工程与生物工程系，浙江杭州 310027;浙江省丽水市环境保护检测中心站，浙江丽水 323000;浙江理工大学生命科学学院，浙江杭州 310018

Abstract

Abstract: A SA/NaCS-CaCl₂/PMCG microcapsule which can be used in biotechnology was prepared.The production of nattokinase from Bacillus subtilis immobilized in SA/CS-CaCl₂/PMCG microcapsule was studied.The effects of PMCG on the growth of Bacillus subtilis were investgated.The growth rate, consumation rate of xylose and production rate of nottokinase of microencapsulated cells were compared with suspension culture.The results showed that immobilized cells grew as suspension culture for a single batch.The encapsulated fermentation had advantage in multi-batch culture.The highest activities of 2465 IU•ml^-1 of nattokinase were reached, which was 2 times higher than that in suspension culture.

Key words: SA/NaCS-CaCl₂/PMCG微胶囊, 固定化, 纳豆激酶

摘要： 制备了海藻酸钠/纤维素硫酸钠-CaCl₂/聚亚基二胍氯化氢微胶囊（SA/NaCS-CaCl₂/PMCG微胶囊），并以该微胶囊固定化培养枯草杆菌HL-1用于发酵制备纳豆激酶，考察了PMCG对枯草杆菌生长的影响，比较了微囊化培养与游离培养过程中的菌体生长、耗糖速率以及纳豆激酶产物分泌能力.最后经过连续6批培养，酶活可达到2465 IU•ml^-1，是游离培养过程的3倍.

关键词: SA/NaCS-CaCl₂/PMCG微胶囊, 固定化, 纳豆激酶

MEI Lehe, ZHANG Xiaozhong, AI Biying, SHENG Qing, LIN Dongqiang, YAO Shanjing, ZHU Ziqiang . IMMOBILIZED CULTURE OF Bacillus subtilis IN SA/CS-CaCl₂/PMCG MICROCAPSULE FOR PRODUCTION OF NATTOKINASE[J]. CIESC Journal, 2004, 55(8): 1319-1323.

梅乐和, 章小忠, 艾碧英, 盛清, 林东强, 姚善泾, 朱自强. SA/NaCS-CaCl₂/PMCG微胶囊固定化枯草杆菌HL-1发酵生产纳豆激酶 [J]. 化工学报, 2004, 55(8): 1319-1323.

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IMMOBILIZED CULTURE OF Bacillus subtilis IN SA/CS-CaCl₂/PMCG MICROCAPSULE FOR PRODUCTION OF NATTOKINASE

SA/NaCS-CaCl₂/PMCG微胶囊固定化枯草杆菌HL-1发酵生产纳豆激酶

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