Abstract: In order to produce macromolecules by using the immobilized cells in microcapsules, a novel macro-porous NaCS-PDMDAAC capsules system was used to immobilize Candida valida in a semi-continuous culture mode for producing lipase. It was found that the encapsulation system had no negative effect on the growth and lipase productivity, and the lipase produced inside capsules could diffuse freely to the media outside capsules. The cultivation with the capsules was carried out for 15 batches and the lipase in each batch had almost the same activity. In comparison with free cell cultivation, the immobilized cell fermentation shortened obviously the period of glucose consumption from 24 hours to 8 hours and increased lipase productivity.

Key words: 脂肪酶, 细胞固定化, 大孔型微胶囊, 纤维素硫酸钠-聚二甲基二烯丙基氯化铵微胶囊, 假丝酵母

关键词: 脂肪酶, 细胞固定化, 大孔型微胶囊, 纤维素硫酸钠-聚二甲基二烯丙基氯化铵微胶囊, 假丝酵母