Abstract: Metabolic flux analysis (MFA) was used to study the relationships among different metabolic pathways in the fermentation of Corynebacterium glutamicum CCTCC M201005.Metabolic network models were established to study the fermentation behavior under different fermentation stages and different dissolved oxygen tension (DOT) levels in batch fermentation.The effects of metabolic node on REA-11 secretion were evaluated.In addition, the supply and consumption of energy and reducing equivalent in cell metabolism were analyzed as well.The results showed that the flux of Phosphate-pentose Pathway (PP) was maintained at a high level during the whole fermentation process, indicating PP played a key role in the metabolism of C. glutamicum CCTCC M201005. The flux of REA-11 decreased when DOT increased, whereas cell synthesis flux showed a reverse trend.Increase of ATP flux stimulated cell growth but decreased REA-11 production.

摘要： 利用代谢通量分析方法，对谷氨酸棒杆菌Corynebacterium glutamicum CCTCC M201005分批发酵不同阶段和不同溶氧浓度下的代谢网络模型进行了计算，考察了代谢节点对絮凝剂REA-11合成的影响，并对C. glutamicum生长代谢过程中能量和还原力的产生及消耗问题进行分析.结果发现，磷酸戊糖途径(PP)通量在整个发酵过程中始终维持在较高的水平;REA-11合成通量随溶氧浓度的增加而降低，菌体合成通量则随溶氧水平的增加而增加;ATP通量的增加可以促进菌体生长，而与REA-11的合成呈负相关.