Abstract: A newly isolated strain was identified as Microbacterium sp. ZD-M2, which can specifically remove sulfur from the model compound of dibenzothiophene (DBT). Metabolites were identified by gas chromatography-mass spectrometry, and the results showed that 2-hydroxybiphenyl, the end product of the previously reported sulfur-specific pathway (also called 4S pathway), was further converted to 2-methoxybiphenyl. Biphenyl was also produced as the deoxidized metabolites. In aqueous phase the strain grew well in the pH range of 6.5—9.5.The optimal temperature for growth was 30 ℃ and the appropriate concentration of ammonium chloride as the source of nitrogen was 1.0 g•L^-1. Glycerol and DBT were selected as the best carbon and sulfur sources for its growth, and the optimal concentrations were 2.0 g•L^-1 and 0.2 mmol•L^-1,respectively.

摘要： 分离得到的微杆菌(Microbacterium sp.)ZD-M2是一株专一性脱硫菌，GC-MS分析表明该菌能选择性地脱除二苯并噻吩(DBT)中的硫，沿“4S途径”代谢的终产物2-羟基联苯(2-HBP)会进一步氧化为2-甲氧基联苯(2-MBP)，同时降解产物中还有联苯（还原产物）存在.在水溶液中降解DBT的结果表明：该菌的适宜生长pH范围为6.5～9.5，最适生长温度为30 ℃，氯化铵为最佳氮源，生长的最佳浓度为1.0 g•L^-1.该菌能利用多种碳源和硫源进行生长，但以甘油和DBT为最佳，最适浓度分别为2.0 g•L^-1和0.2 mmol•L^-1.