Abstract: The effect of trehalose on the refolding of denatured lysozyme was investigated at different guanidine hydrochloride（GdnHCl）concentrations.The competitive model of first-order folding and third-order aggregation could well describe the dynamic refolding process in the presence of trehalose.It was found that aggregation rate constant k_A decreased obviously by adding trehalose，but the folding rate constant k_N was little affected.At a proper concentration of GdnHCl，k_A decreased first and then increased with increasing trehalose concentration.At the optimal trehalose concentration，the aggregation rate constant k_A reached a minimum，while the ratio of k_N/k_A reached a maximum correspondingly.Thus，at this additive concentration a maximum refolding yield was obtained.The result indicated that the main role of trehalose was to prevent intermolecular aggregation.Therefore，in a proper range of concentration，increasing trehalose concentration could facilitate refolding of protein and increase refolding yield.

摘要： 研究了海藻糖促进变性还原溶菌酶的复性作用，考察了不同盐酸胍浓度下海藻糖浓度对复性收率的影响，并利用表观竞争反应动力学模型分析了海藻糖辅助溶菌酶的复性动力学特性.结果表明，在海藻糖存在下，聚集体生成速率常数k_A明显减小，但复性速率常数k_N变化不大;随海藻糖浓度的增大，k_A先降低后升高，在合适浓度时k_A出现最小值，由于此时k_N/k_A出现最大值，因此复性收率最大.说明海藻糖的主要作用是有效抑制蛋白质分子间聚集，因此在一定范围内提高其浓度可促进蛋白质复性，从而提高复性收率.