Abstract: Three kinds of support for enzyme immobilization were prepared by modifying SBA-15 mesoporous silica with thiol，methyl and hexyl groups respectively.The prepared supports were characterized with FT-IR and BET method and exhibited high specific surface area and uniform pore size.Furthermore，unmodified SBA-15 and the modified SBA-15 materials were used for lipase immobilization.The results were compared in terms of enzyme adsorption capacity，activity，activity retention and stability.The modified SBA-15 materials with thiols（SBA-15-PrSH）showed good performance for lipase immobilization.Compared with unmodified SBA-15，the adsorption capacity of SBA-15-PrSH to lipase was improved by more than 80%，and the activity retention increased by more than 10%.