Abstract: Laccase from Coriolus versicolor was immobilized by adsorbing first on activated carbon, and then entrapping into calcium alginate gels. 2,4-Dichlorophenol in wastewater was dechlorinated by the immobilized laccase in a columnar reactor. The optimal temperature and pH for the reaction were 40℃ and 4.5, respectively. Compared with free laccase, the immobilized laccase had better thermostability, and also had a broader active range of pH and temperature. The decholorination efficiency of laccase on 2,4-dichlorophenol (1 mmol•L^-1) was all above 99.5% with 0.5 mmol•L^-1 chloride ions released into the water during 8 repeated batch reactions. The results showed good prospect of application in the biochemical treatment of polluted wastewater.

摘要： 采用活性炭吸附与海藻酸钙凝胶包埋相结合的方法使Coriolus versicolor漆酶固定化.利用固定化漆酶对2，4-二氯酚进行脱氯反应，其最适pH值为4.5、最适温度为40℃.与游离酶相比，固定化酶反应的pH值和温度范围更宽，其稳定性得到了明显改善.使用柱式固定化酶反应器处理2，4-二氯酚，在批式反应工艺条件下，当底物浓度为1 mmol•L^-1、反应3～5 h, 2，4-二氯酚的去除率可达99.5%以上（脱除的氯离子浓度达0.5 mmol•L^-1）.连续8批反应的结果表明：固定化漆酶性能稳定、催化效率高，在环境污染废水治理方面具有良好的应用前景.