Abstract: Separation of fermentation broth of lincomycin was studied with assembled continuous flocculation and jar testing.The experimental results of jar testing showed that the best concentration of flocculant cationic polyacrylamide in the broth was 70 mg•L^-1.After flocculation, the precipitation rate of the flocs was 24% larger and the filtration rate of the broth was 31% larger by cationic polyacrylamide than by oxalic acid used in the industry.The experimental course of assembled continuous flocculation was modified jar testing，in that the broth was conveyed by a pump and pipeline from fermentation pot to a mixer，in which cationic polyacrylamide and the broth were mixed.Afterward the broth was flocculated continuously in the column，and then the flocs were separated from the broth by filtration or centrifugal precipitation.In the continuous flocculating column，the flux of the broth was 1 L•min^-1，average stay time was 10 min，pH was 3，temperature was 20℃，the concentration of cationic polyacrylamide was 70 mg•L^-1.The experimental results were as follows：the precipitation rate of the flocs in the broth was 46% larger and the filtration rate of the broth was 67% larger in assembled continuous flocculation than in jar testing.The results offer important evidence for flocculated fermentation broth to be separated with high efficiency by filtration or centrifugal precipitation in the industry.

摘要： 用组合连续絮凝法及容器实验絮凝法对林可霉素发酵液进行絮凝分离研究.容器实验絮凝法结果显示：阳离子型聚丙烯酰胺絮凝分离效果优于壳聚糖和草酸，其最佳浓度为70 mg•L^-1，此时絮体沉降速率比用草酸时高24%，过滤速率高31%.组合连续絮凝法是对容器实验絮凝法的改进，其实验路线为：泵和管道输送发酵液→絮凝剂和发酵液在混合器中混合→絮凝柱连续流动絮凝→分离器固液分离.絮凝柱流量1 L•min^-1，平均停留时间10 min，pH为3，温度20℃，阳离子型聚丙烯酰胺浓度70 mg•L^-1.结果显示：组合连续絮凝法比容器实验絮凝法的沉降速率提高46%，过滤速率提高67%.该结果为工业发酵液高效过滤分离及高效离心分离提供了重要依据.