Abstract: ε-Poly-L-lysine(ε-PL) was isolated and purified from the culture broth of Kitasatospora sp.PL6-3.Through the ion exchange process and Sephadex G-25 gel filtration chromatography,a high purity product was obtained with overall yield of 81.2%.The purified ε-PL was characterized with UV, IR and HR/MAS NMR.Its relative molecular weights as determined by SDS-PAGE and gel permeation chromatography (GPC) were about 5.01×10³ and 5.05×10³ respectively, which was higher than the other ε-PL reported before.

摘要： 从北里孢菌（Kitasatospora sp.）PL6-3的发酵液中分离纯化ε-聚赖氨酸（ε-PL），首先发酵液经离子交换，得到ε-PL粗品，然后通过凝胶过滤Sephadex G-25柱层析，获得了色谱纯的产品，分离总收率为81.2%.利用紫外吸收光谱、红外光谱（IR）和高分辨率魔角旋转核磁共振谱 （HR/MAS NMR），确认了该聚合物的结构.用SDS-PAGE电泳和凝胶渗透色谱（GPC）测得ε-PL的相对分子质量分别为5.01×10³和5.05×10³，略高于文献报道的其他菌所产的ε-PL.