• 生物化学工程、制药、食品和天然产物加工 • Previous Articles     Next Articles

Identification and characterization of a PTA-degrading bacterial strain

FENG Yangyang;CHEN Jun;LIU Bo;CHEN Yingwen;ZHENG Guoyang;LU Jianhua;SHEN Shubao
  

  • Online:2006-08-25 Published:2006-08-25

一株对苯二甲酸降解菌的鉴定及其降解特性

冯杨阳;陈俊;刘波;陈英文;郑国洋;陆建华;沈树宝   

  1. 南京工业大学工业生物技术研究所,江苏 南京 210009;中国石油化工股份有限公司扬子石油化工责任有限公司,江苏 南京 210048

Abstract: A strain PA-18 was isolated from the active sludge of p-phthalic acid (PTA) wastewater treatment in Yangtze Petrochemical Company.The 16SrDNA fragment was amplified from strain PA-18 by PCR,and the 16SrDNA sequence analysis indicated that strain PA-18 shared 99% 16SrDNA sequence homology with Pseudomonas sp..In the phylogenetic framework of bacterial classification,PA-18 belongs to Pseudomonas sp..Under the condition of temperature 37℃,pH 7.0 and OD660 nm2.0,the PTA decomposition rate of this bacterial strain was beyond 95% after 24 h.Meanwhile,this strain also could treat PTA wastewater with CODCr removal of 85%.p-Phthalic acid was degraded by the strain PA-18 through the intermediate formation of protocatechuic acid in the aerobic state.

摘要: 从扬子石油化工责任有限公司废水处理装置的污泥池中分离、筛选得到了一株高效降解对苯二甲酸(PTA)的菌株,PA-18.采用PCR扩增获得该菌16SrDNA片段,核苷酸序列分析结果表明:该菌株的16SrDNA的核苷酸序列与多株假单胞菌(Pseudomonas sp.)的同源性为99%,在细菌系统发育分类学上,该菌株属于假单胞属.在温度为37℃、pH 7.0、OD660 nm2.0条件下,反应24 h,此菌对PTA的降解率大于95 %.同时对于实际的PTA废水也有很好的处理效果,CODCr去除率高达85%.在好氧降解作用下,PTA降解的主要中间物被确定为原儿茶酸.