Abstract: The phase behavior of Triton X-100-salt aqueous two-phase systems was studied at 298 K.In consideration of the contributions of the sorption effect of the micelles, a theoretical model was established to describe the phase behavior of Triton X-100-salt aqueous two-phase systems and the distribution equilibrium of alkaline protease in the systems.Experimental results demonstrated the reliability and usefulness of the model.It was found that the model was suitable for neutral systems.Non-ionic surfactant combined easily with ions in the solution, thus influencing the behavior of the systems.Hydrophobicity of alkaline protease enhanced by salts in the systems increased the dissolution of protease.

摘要： 研究了Triton X-100-无机盐双水相萃取体系在298 K时的相行为，建立了包含界面吸附效应的非离子表面活性剂双水相液-液平衡模型及碱性蛋白酶在其中的分配系数模型，验证了模型的可靠性和适用性.研究发现，该模型对中性范围的非离子表面活性剂双水相萃取体系的相行为及碱性蛋白酶分配平衡的模拟效果较好;非离子表面活性剂在水溶液中由于易结合离子而影响相行为，使体系更易分相;无机盐增大了碱性蛋白酶的表面疏水性，使碱性蛋白酶在疏水相中增溶.