Abstract:

In order to obtain lactic acid bacteria using molasses wastewater to produce lactic acid，an activated sludge was sampled from bioreactor with molasses wastewater，and strain MD-9 was isolated by an anaerobic Hungate technique. The morphology，physic-biochemical characteristics and 16S rRNA sequence analysis of strain MD-9 were investigated. The results indicated that 16S rRNA gene homology of strain MD-9 with Lactobacillus fermentum was 99. 6%.Strain MD-9 is facultative anaerobe bacillus. Glucose fermentation is heterologous. Strain MD-9 can use molasses wastewater to produce lactic acid.

摘要： 为获得可利用糖蜜废水产生乳酸的细菌，采用厌氧Hungate培养技术，从糖蜜废水处理生物反应器中获得活性污泥，分离到一株乳酸菌MD-9。对该株细菌进行了形态学特征、生理生化指标、16S rRNA基因序列分析等研究。结果表明与最相近的种属Lactobillus fermnetus的16S rRNA基因序列同源性为99.6%。该株细菌为兼性厌氧杆菌，葡萄糖代谢特征为专性异型发酵，可以利用糖蜜废水产生乳酸。