Abstract:

A simple UV spectroscopic method for determination of sugars was developed.Calibration models were established by the spectral measurement of a set of hydrolyzed standard sugar solutions by treating with concentrated sulfuric acid and a chemometrical software.The sugar contents in the sample can be predicted with the models by using the multi-wavelength spectral signals of its hydrolyzed solution.The present method does not need organic reagents and can provide the information of pentoses, hexoses, and total sugars in samples, and is suitable to be used for sugar analysis of the lignocellulosic biomass extraction process in bio-refinery research.

Key words:

紫外光谱, 混合糖, 化学计量学, 生物炼制, 木质生物质

摘要：

提出一种用紫外光谱测定样品中混合糖含量的方法。其原理是基于样品中的糖经浓硫酸处理后生成有紫外吸收的糠醛和羟甲基糠醛，进行紫外波长吸收测定;通过化学计量学软件，根据一组已知糖浓度标准样品的多波长光谱和所对应组成浓度之间的数学关系，建立校正模型。将未知样品的紫外光谱信息输入校正模型即可预测其组成中糖的含量。本方法无需使用有机试剂，操作简便，可以测定样品中戊糖、己糖和总糖的含量，适合于木质生物质炼制研究中溶解性糖类组分的分析。

关键词:

紫外光谱, 混合糖, 化学计量学, 生物炼制, 木质生物质