Abstract:

The effects of acid hydrolysis pretreatment of corn cob on saccharification efficiency and fermentation bio-hydrogen production were investigated by orthogonal test design.The hydrolysis pretreatment of corn cob with dilute sulfuric acid was essential for adequately converting substrate into soluble saccharides and bio-hydrogen.The experimental results showed that acid hydrolysis pretreatment of corn cob was found most effective at hydrolysis temperature of 115℃, sulfuric acid concentration of 1.0%, hydrolysis time of 1.5 h and solid-liquid mass ratio 1∶10, in which both maximum saccharification efficiency of 0.5433 g·（g TVS）^-1 and bio-hydrogen yield of 85.8 ml H₂·(g TVS)^-1 were observed.In addition, the mechanisms of degrading corn cob by acid hydrolysis and hydrogen fermentation were also discussed by means of X-ray diffraction（XRD）analysis and crystallinity calculation.

Key words:

玉米芯, 酸水解, 发酵, 生物产氢

摘要： 通过正交实验研究了硫酸水解预处理对玉米芯糖化效率及发酵产氢的影响，优化了相应的工艺参数，结果表明：玉米芯的最佳糖化效率和发酵产氢出现在水解温度115℃ ，硫酸浓度1.0%，水解时间1.5 h和固液比(M/V)l:10，在该条件下，玉米芯糖化效率为0.5433g·（g TVS）^-1，酸解残渣的氢产氢达 85. ml H₂·(g TVS)^-1。此外，通过X射线粉末衍射分析和结晶度计算探讨了酸解预处理和氢发酵对玉米芯的作用机理。

关键词:

玉米芯, 酸水解, 发酵, 生物产氢