Abstract:

The study was based on the principle of shortcut denitrifying phosphorus removal.In order to enrich shortcut denitrifying phosphorus removing bacteria (SDPB), anaerobic sludge was used in the anaerobic/anoxic SBR as inoculum.Anaerobic/anoxic phase was studied and nitrite was added as electronic acceptor.A SDPB strain was screened and isolated and the classification status was studied by adopting traditional and modern molecular identification methods.The biological utilization of the bacterial strain under different nutrition and environment conditions was investigated.The result showed that the bacterium was facultative anaerobe and bore simultaneous shortcut denitrifying/phosphorus removing function.It belonged to Acinetobacter sp.according to its morphological, physiology, biochemical, properties and the analysis of 16S rDNA gene sequence with 99.3% similarity.The optimum carbon resource was sodium acetate.The bacteria used not only nitrite but also nitrate as electronic acceptor.The best pH value was 7 and the best temperature was 25℃.The appropriate temperature was 20—35℃.The growth of micro-organism was inhibited when the temperature was 10℃.The activity of microorganism decreased when the temperature was above 35℃.The highest removal efficiency of phosphate and nitrate were 82.94% and 82.99%,respectively.

Key words:

反硝化除磷, 短程反硝化除磷菌, 亚硝酸盐

摘要：

依据短程反硝化除磷原理，在SBR装置中加入厌氧池污泥，采用厌氧/缺氧工艺，投入亚硝酸盐以富集短程反硝化除磷菌（SDPB），并进行SDPB的筛选、分离，采用传统与现代分子生物学鉴定相结合手段确定其分类地位，同时进行不同营养条件和环境条件下菌的生物学利用研究。结果表明：该菌株为一株新的兼性厌氧菌株，具有同步短程反硝化和除磷功能。通过细菌形态、生理生化指标、培养特征和16S rDNA序列进行同源性比较，鉴定该菌株为不动杆菌属，相似性高达99.3%，该种尚未见文献报道。Gi菌的最佳碳源为乙酸钠。此菌不仅可以利用NO^-₂也可以利用NO^-₃为电子受体。Gi菌的最佳pH值为7。温度为25℃时的反硝化除磷效果最好，适宜的温度为20～35℃。当温度为10℃时微生物生长受到抑制，温度高于35℃时活性下降。磷、氮的最高去除率分别可达82.94%和82.99%。

关键词:

反硝化除磷, 短程反硝化除磷菌, 亚硝酸盐