Abstract:

Hydrothermal pretreatment（195 ℃，15 min）and simultaneous saccharification and fermentation（SSF）were adopted to produce ethanol from corn stover. Results showed that 86.5% of cellulose was remained in the solid cake and most of hemicellulose was solubilized after the hydrothermal pretreatment. The highest ethanol concentration of 12.12 g/ L was achieved at initial pH of 5.5，temperature of 30 ℃ and 130 r/min shaking rate with substrate concentration of 50 g/L by Pichia stipitis after 192 h. The corresponding alcohol yield and volumetric productivity were 0.34 g/g（glucose + xylose）and 0.065 g/(L·h) respectively.

摘要：

采用湿热预处理（195 ℃，15 min）与同步糖化发酵对玉米秸秆制备酒精进行了研究。结果表明：玉米秸秆经过湿热预处理后，86.5%纤维素保留在滤饼中，而大部分半纤维素被溶解。在底物质量体积含量50 g/L，温度30 ℃，pH值5.5，摇床转速130 r/min条件下，树干毕赤酵母（Pichia stipitis）利用预处理后的玉米秸秆经过192 h同步糖化发酵，酒精浓度达到了12.12 g/L，对应的酒精产量和生产效率分别为0.34 g/g（葡萄糖+木糖）和0.065 g/(L·h)。该项研究为工业化生产打下了基础。