Abstract:

Extraction and separation of phycocyanin from nostoc commune supernatant was investigated with reversed micellar extraction by using cationic surfactant CTAB. Effects of pH value in aqueous phase，ionic strength and ion type，volume ratio of solvent in organic phase，as well as concentrations of surfactant and co-solvent on the extraction behavior were respectively evaluzted. Orthogonal experiments were conducted to investigate the effects of the extractant type，concentration and pH value on the back extraction behavior，and finally optimized back extraction conditions were obtained. The partition coefficient of phycocyanin was found as high as 50.7 with 98.1% extraction yield by 0.05 mol/L CTAB/n-hexanol-octane（1∶4，v/v）from the supernatant with pH 7.0. Different ion type and intensity showed different effect on extraction rate. The extraction rate decreased with the increase in ionic strength. The back extraction yield of phycocyanin was 98.5% by 3 mol/L KBr solution with pH4.0 as back extractant，and the purity of phycocyanin was 16.8.

摘要：

采用阳离子表面活性剂十六烷基三甲基溴化铵（CTAB）反胶团萃取分离地木耳中藻蓝蛋白。分别考察水相pH值、离子强度和种类、有机相中有机溶剂配比、表面活性剂浓度和助溶剂浓度对萃取行为的影响；同时采用正交试验法，考察反萃取过程中反萃液种类、浓度和pH值对萃取液中藻蓝蛋白反萃取行为的影响，最终得到适宜的萃取分离工艺条件。结果表明：0.05 mol/L CTAB/正己醇-正辛烷（体积比1∶4）的反胶团体系用于萃取pH=7的地木耳细胞破碎液，藻蓝蛋白萃取率可达 98.1%，分配系数达到50.7；溶液中不同离子种类和强度对萃取率影响不同，萃取率随着离子强度增大而降低；采用pH=4.0、3 mol/L KBr反萃液反萃藻蓝蛋白，反萃率可达98.5%，其纯度可达16.8。