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GUO Feige,ZHANG Qiuyu,ZHANG Hepeng,ZHANG Baoliang
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郭飞鸽,张秋禹,张和鹏,张宝亮
Abstract:
Magnetic composite microspheres of Fe3O4/P(AA-MMA-GMA)were prepared via emulsifier-free emulsion polymerization of acrylic acid(AA),methyl methacrylate(MMA)and glycidyl methacrylate(GMA)using 1,1-diphenylethlene as free radical control agent. The obtained Fe3O4/P(AA-MMA-GMA)microspheres were then used to prepare immunomagnetic microspheres via the reaction with an antibody of goat anti-rabbit IgG. The Fe3O4/P(AA-MMA-GMA)microspheres showed superparamagnetism behavior with particle size of 626 nm bearing epoxy groups on the surface. The immunomagnetic microspheres were obtained by reacting 0.5 mg of Fe3O4/P(AA-MMA-GMA)with 200 μg of goat anti-rabbit IgG in a phosphate buffer solution with pH 7.4 at 25 ℃ for 16 h,the coupling quantity for goat anti-rabbit IgG was 124 μg. The immunomagnetic microspheres can be specifically bound to rabbit anti-actin alpha.
摘要:
采用新型的无皂乳液聚合法制备磁性复合微球,即以丙烯酸(AA)、甲基丙烯酸甲酯(MMA)和甲基丙烯酸缩水甘油酯(GMA)为单体,1,1-二苯基乙烯为自由基控制剂,无皂乳液聚合制备了一种磁性复合微球Fe3O4/P(AA-MMA-GMA)。以所制备的Fe3O4/P(AA-MMA-GMA)微球为载体,通过共价偶联山羊抗兔IgG抗体,得到了一种免疫磁性复合微球。研究表明,Fe3O4/P(AA-MMA-GMA)微球表面带有环氧基,粒径为626 nm,具有超顺磁性。0.5 mg Fe3O4/P(AA-MMA-GMA)微球与200 μg山羊抗兔IgG抗体在pH=7.4的磷酸盐缓冲液中于25 ℃反应16 h,可得到一种免疫磁性复合微球,其山羊抗兔IgG的偶联量为124 μg,且该免疫磁性复合微球可特异性结合兔抗肌动蛋白α。
GUO Feige,ZHANG Qiuyu,ZHANG Hepeng,ZHANG Baoliang. Fe3O4/P(AA-MMA-GMA)magnetic composite microspheres,[J]. .
郭飞鸽,张秋禹,张和鹏,张宝亮. Fe3O4/P(AA-MMA-GMA) 磁性复合微球的制备及其 [J]. CIESC Journal.
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https://hgxb.cip.com.cn/EN/Y2010/V29/I9/1693