Abstract:

Based on the principle of simultaneously denitrifying and short-cut sulfide removing，monosodium glutamate waste water sludge was used as inoculum，and the continuous stirred reactor was used to enrich the simultaneously denitrifying and short-cut sulfide removing bacteria.Traditional and modern molecular biology methods were adopted to determine the taxonomic status of the isolated bacteria which was named SNB.Response surface methodology was used to optimize the growth condition.The result showed that SNB had 99.0% homology with Thauera selenatis.Morphological and biochemical characteristics were also similar to Thauera selenatis.It belonged to short rod species.Thauera selenatis is not named in Chinese yet.Biochemical properties，stirred reactor reaction conditions and material balance showed that SNB was a kind of facultative anaerobic denitrifying and sulfide removing bacteria.The first and second items of pH were 0.0038 and 0.0095 respectively.The first and second items of peptone were 0.0868 and 0.447 respectively.The value of pH had a significant effect on the growth of SNB，peptone was the second.The best growth conditions were pH 8，the addition of peptone and sucrose was 1.88 g·L^-1 nd 30.0 g·L^-1 respectively.

Key words:

反硝化短程除硫, 反硝化除硫菌, 响应面分析法, 生长条件

摘要：

依据同步反硝化除硫原理，以味精废水污泥为种泥，利用全混流反应器富集并分离出同步反硝化短程除硫菌（SNB），采用传统与现代分子生物学相结合的手段对其鉴定，以确定其分类地位;利用响应面分析法优化SNB的主要生长条件。结果表明：SNB与Thauera selenatis同源性达99.0%，形态特征及生理生化与Thauera selenatis最相似，属短杆菌属，Thauera selenatis尚无中文命名;生理生化指标、全混流反应器反应条件及物料平衡显示SNB是一株兼性厌氧反硝化除硫菌;pH一次项、二次项的数值分别为0.0038、0.0095，蛋白胨一次项、二次项的数值分别为0.0868、0.447，pH对SNB菌生长有显著影响，蛋白胨对SNB生长影响其次;三因素交互影响结果为pH值最优为8，蛋白胨和蔗糖的最优投加量分别为1.88 g·L^-1和30.0 g·L^-1。

关键词:

反硝化短程除硫, 反硝化除硫菌, 响应面分析法, 生长条件