Optimization of fermentation conditions in liquid state of Trichoderma viride to produce yellow pigment

CIESC Journal ›› 2010, Vol. 61 ›› Issue (12): 3205-3212.

Previous Articles Next Articles

Optimization of fermentation conditions in liquid state of Trichoderma viride to produce yellow pigment

ZHANG Xiaoyi,CUI Yingde, LU Ning, YU Xin, ZHAO Meimei

Online:2010-12-05 Published:2010-12-05

绿色木霉菌产黄色素液态发酵条件的优化

张晓毅，崔英德，陆宁，于新，赵美美

安徽农业大学茶与食品科技学院，安徽合肥 230036;仲恺农业工程学院轻工食品学院，广东广州 510225

Abstract

Abstract:

The composition of nutrient medium and the condition of fermentation process were optimized for T.viride producing yellow pigment by three-factor Box-Behnken design and response surface analysis, which was based on the experimental results of single factor for testing bacterial strain Trichoderma viride,including the source of carbon and nitrogen, liquid loading, rotation speed, inoculum concentration, culture time and temperature etc.The results show that the optimum culture medium components and conditions are: liquid culture medium based on PDA(250 g·L^-1 potato extract and 2 g·L^-1 Na₂HPO₄), 35℃, inoculum 6%(vol), carbon source 21.7 g·L^-1 xylose, loading liquid amount 50 ml in 250 ml Erlenmeyer flask and culture time 152 h at 120 r·min^-1. In the optimal conditions, the color value of yellow pigment obtained is (52.8±0.8)U·ml^-1.The formation of yellow pigment would be inhibited by adding urea, ammonium sulfate, peptone, beef extract powder as the source of nitrogen.

Key words: FONT-SIZE: 10.5pt, mso-bidi-font-size: 11.0pt, mso-bidi-font-family: 宋体, mso-font-kerning: 1.0pt, mso-ansi-language: EN-US, mso-fareast-language: ZH-CN, mso-bidi-language: AR-SA, mso-ascii-font-family: Calibri">绿色木霉菌hansi-font-family: 宋体" lang=EN-US>;FONT-SIZE: 10.5pt, mso-bidi-font-size: 11.0pt, mso-bidi-font-family: 宋体, mso-font-kerning: 1.0pt, mso-ansi-language: EN-US, mso-fareast-language: ZH-CN, mso-bidi-language: AR-SA, mso-ascii-font-family: Calibri">培养基组成hansi-font-family: 宋体" lang=EN-US>;FONT-SIZE: 10.5pt, mso-bidi-font-size: 11.0pt, mso-bidi-font-family: 宋体, mso-font-kerning: 1.0pt, mso-ansi-language: EN-US, mso-fareast-language: ZH-CN, mso-bidi-language: AR-SA, mso-ascii-font-family: Calibri">液态发酵条件hansi-font-family: 宋体" lang=EN-US>;FONT-SIZE: 10.5pt, mso-bidi-font-size: 11.0pt, mso-bidi-font-family: 宋体, mso-font-kerning: 1.0pt, mso-ansi-language: EN-US, mso-fareast-language: ZH-CN, mso-bidi-language: AR-SA, mso-ascii-font-family: Calibri">黄色素

摘要：

以绿色木霉菌(Trichoderma viride)为试验菌株，在培养基碳源、氮源组成，装液量，摇床转速，接种量，培养时间及培养温度等单因素试验的基础上，采用Box-Behnken设计及响应面分析法优化T.viride产天然黄色素的培养基组成和发酵工艺条件。结果表明，在试验范围内，T.viride产黄色素的优化培养条件为：马铃薯液态培养基（250 g·L^-1马铃薯浸汁，2 g·L^-1Na₂HPO₄），外加碳源选择21.7 g·L^-1木糖，摇瓶装液量为50 ml·（250 ml）

关键词: FONT-SIZE: 10.5pt, mso-bidi-font-size: 11.0pt, mso-bidi-font-family: 宋体, mso-font-kerning: 1.0pt, mso-ansi-language: EN-US, mso-fareast-language: ZH-CN, mso-bidi-language: AR-SA, mso-ascii-font-family: Calibri">绿色木霉菌hansi-font-family: 宋体" lang=EN-US>;FONT-SIZE: 10.5pt, mso-bidi-font-size: 11.0pt, mso-bidi-font-family: 宋体, mso-font-kerning: 1.0pt, mso-ansi-language: EN-US, mso-fareast-language: ZH-CN, mso-bidi-language: AR-SA, mso-ascii-font-family: Calibri">培养基组成hansi-font-family: 宋体" lang=EN-US>;FONT-SIZE: 10.5pt, mso-bidi-font-size: 11.0pt, mso-bidi-font-family: 宋体, mso-font-kerning: 1.0pt, mso-ansi-language: EN-US, mso-fareast-language: ZH-CN, mso-bidi-language: AR-SA, mso-ascii-font-family: Calibri">液态发酵条件hansi-font-family: 宋体" lang=EN-US>;FONT-SIZE: 10.5pt, mso-bidi-font-size: 11.0pt, mso-bidi-font-family: 宋体, mso-font-kerning: 1.0pt, mso-ansi-language: EN-US, mso-fareast-language: ZH-CN, mso-bidi-language: AR-SA, mso-ascii-font-family: Calibri">黄色素

ZHANG Xiaoyi, CUI Yingde, LU Ning, YU Xin, ZHAO Meimei. Optimization of fermentation conditions in liquid state of Trichoderma viride to produce yellow pigment[J]. CIESC Journal, 2010, 61(12): 3205-3212.

张晓毅, 崔英德, 陆宁, 于新, 赵美美. 绿色木霉菌产黄色素液态发酵条件的优化 [J]. 化工学报, 2010, 61(12): 3205-3212.

[1]	JIANG Fangyan1, WANG Jinmei2, DAI Dazhang1, LI Chun1,2. Screening and identification of phospholipase-producing strains and their applications [J]. CIESC Journal, 2012, 63(3): 887-893.
[2]	. [J]. CIESC Journal, 2011, 62(S1): 42-47.
[3]	. [J]. CIESC Journal, 2011, 62(S1): 48-53.
[4]	. [J]. CIESC Journal, 2011, 62(S1): 60-65.
[5]	. [J]. CIESC Journal, 2011, 62(S1): 92-96.
[6]	. [J]. CIESC Journal, 2011, 62(S1): 103-107.
[7]	. [J]. CIESC Journal, 2011, 62(S1): 108-112.
[8]	. [J]. CIESC Journal, 2011, 62(S1): 113-117.
[9]	. [J]. CIESC Journal, 2011, 62(S1): 168-173.
[10]	. [J]. CIESC Journal, 2011, 62(S1): 157-162.
[11]	. [J]. CIESC Journal, 2011, 62(S1): 146-150.
[12]	. [J]. CIESC Journal, 2011, 62(S1): 130-133.
[13]	. [J]. CIESC Journal, 2011, 62(S1): 197-202.
[14]	MA Youfu，YUAN Yichao，CHEN Yu, LIU Hailei，KANG Ming. Effects of fin pitch on heat transfer and flow resistance of serrated spiral-finned-tube banks [J]. , 2011, 62(9): 2484-2489.
[15]	LIU Ge，XIE Maozhao，JIA Ming. Large eddy simulation of combustion processes in ICE based on RIF model [J]. , 2011, 62(9): 2490-2498.

Optimization of fermentation conditions in liquid state of Trichoderma viride to produce yellow pigment

绿色木霉菌产黄色素液态发酵条件的优化

PDF (PC)

Knowledge

Cited

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments