Abstract:

Adsorbent resin JD-1 was used for the in situ product removal (ISPR) in the pristinamycin production with S. pristinaespiralis E-71. A kinetic model for pristinamycin production and the film-pore diffusion model (FPDM) were combined together to describe the ISPR process in a 2. 5 L stirred tank bioreactor. Model parameters including pristinamycin formation rate constant, product inhibition constant, film mass transfer coefficient and pore diffusion coefficient were determined with the nonlinear least squares method. Theoretical calculations indicated that the model was in good agreement with the practical ISPR process. The effect of the amount and size of the resin on the ISPR results was studied. Experimental results showed that up to 90% of the pristinamycin produced by the fermentation could be recovered by the adsorbent resin, and that the performance got better with the decrease of resin size as well as with the increase of resin amount in a specific range. A yield of 1. 01 g·L^-1 was obtained with 95% adsorbed in situ when 70 g·L^-1 resin (R=0.195 mm) was added.

摘要：

根据S. pristinaespiralis E-71普那霉素发酵过程的代谢特性提出了一个针对产物合成期的普那霉素合成动力学方程，结合液膜-孔扩散理论，建立了描述2.5 L通气搅拌发酵罐中JD-1大孔吸附树脂吸附分离原位耦合发酵过程的动力学模型，并获得产物生成速率常数、产物抑制常数及液膜传质和孔内扩散系数。结果表明该发酵-吸附耦合过程动力学模型能较好地描述这一原位分离耦合发酵生产普纳霉素过程。在此基础上，考察了树脂添加量及树脂半径对原位分离耦合发酵过程的影响，结果表明添加JD-1树脂能有效移走发酵液主体相中高达90%的普那霉素，且原位分离效果随树脂粒径减小而增强，在一定范围内随树脂添加量的增加而增强，当添加70 g·L^-1半径为0.195 mm的树脂时，普纳霉素产量达到1. 01 g·L^-1，其中被树脂吸附的高于95%。