Abstract:

Chloroperoxidase（CPO），a highly effective biological catalyst，has very good catalytic activity for an exceptional diversity of reactions，such as halogenations，oxidation，epoxidation，and hydroxylation reactions.A CPO-DDAB/GC modified electrode was prepared by the immobilization of CPO on the surface of a Nafion-coated GC electrode using didodecyldimethylammonium bromide（DDAB）.A pair of well-defined redox peaks with a ΔEp of 80mV was observed for the voltammogram of the CPO-DDAB/GC modified electrode，which suggests a quasi-reversible direct electrode transfer between CPO and the electrode.Further electrochemical studies show that CPO-DDAB/GC modified electrode possesses an excellent electrocatalytic activity for reduction of O₂.The CPO-DDAB/GC modified electrode was also used for electrocatalytic oxidation of toluene.GC-MS analyses demonstrate the formation of benzaldehyde for the oxidation of toluene under the current experimental conditions.

摘要：

氯过氧化物酶是一种高效的生物催化剂，具有良好的催化性能，并且能催化多种反应，例如卤化、氧化、环氧化和醇氧化。利用双十二烷基二甲基溴化铵（DDAB）将氯过氧化物酶（CPO）固定到经Nafion预处理的玻碳电极上，氮气饱和的磷酸缓冲溶液中测得的循环伏安曲线上可见一对氧化还原峰，峰电位差（ΔEp）约为80 mV，氧化还原峰电流之比接近1，表明CPO发生了准可逆的电子传递反应。CPO-DDAB/GC修饰电极对氧还原反应具有显著的电催化效应。利用该修饰电极考察了对甲苯氧化的催化，气质联用（GC-MS）的分析结果表明，目前的实验条件下，能催化甲苯氧化为苯甲醛。