Abstract:

Femtosecond laser induced micro-organisms mutation has become a new microbial technology，because of its characteristics of ultrafast pulse，high peak power and focusing on a small size. In this paper，compared to the advantages and disadvantages of the traditional low-power He-Ne laser irradiation，the technology of femtosecond laser induced micro-organisms mutation is discussed. Meanwhile，compared to traditional laser mutation mechanism of micro-organisms，the mechanisms of multi-photon absorption，formation of plasma，formation of reactive oxygen species，DNA damage and self-repair are summarized. Finally，the prospect for technology of femtosecond laser induced micro-organisms mutation is given，and the combination of key-enzyme activity measurement and dynamics parameters determination is important in choosing the technological parameters of femtosecond laser induced micro-organisms mutation.

摘要：

飞秒激光诱变微生物是一项崭新的技术。本文针对传统低功率He-Ne激光诱变微生物的优点和缺点，结合飞秒激光光束脉冲持续时间短、瞬时功率大、聚焦尺寸小的优点，简要概括了国内外对飞秒激光诱变微生物技术的研究。同时对飞秒激光辐射微生物过程中多光子吸收、形成等离子体、产生生物活性氧、DNA损伤自身修复等一系列的机理研究进行了总结。最后，对飞秒激光诱变微生物技术及其机理研究进行了展望并提出了建议：结合关键酶活性测定和动力学参数测定来选择飞秒激光诱变的技术参数。