Abstract:

Poly 4-vinyl pyridine/SiO₂ (P4VP/SiO₂), a grafted functional particles, was prepared by grafting 4-vinyl pyridine (4VP)on micro-sized silica gel particles with the “graft from” method, using 3-methacryloxypropyl trimethoxysilane (MPS)as coupling agent. Subsequently, by using the novel surface-molecular imprinting technique, the L-Glu molecule-imprinted material MIP-P4VP/SiO₂ was prepared by imprinting towards the grafted P4VP with L-Glu, one enantiomer of glutamic acid (Glu), as template molecule and 1,6-dibromohexane as crosslinking agent. The recognition characteristics of MIP-P4VP/SiO₂ for L-Glu was studied by both static method and dynamic method in which D-Glu was used as a contrast compound. The separation ability of MIP-P4VP/SiO₂ towards L-Glu and D-Glu in the racemic solution was examined. The experiment results showed that MIP-P4VP/SiO₂ had obvious recognition selectivity and fine binding affinity for L-Glu, and the selectivity coefficient was equal to 3.38 with respect to D-Glu, displaying the fine separation property for the enantiomers of glutamic acid. Besides, MIP-P4VP/SiO₂ had excellent elution property with diluted aqueous solution of NaOH as eluent, the desorption ratios of L-Glu could reach 99.53% in 12 BV. Moreover, this material showed effective separation ability of repeated use for more than ten times.