Abstract:

In order to eliminate the bacteria in inoculating sludge, which consume hydrogen, and to speed start-up as well as to rise efficiency of hydrogen production, effect of pretreatment temperatures of 65, 80, 95, 110 and 121℃ for 30 min on hydrogen production from glucose was investigated in a batch cultures, with anaerobic granular sludge come from workshop treating wastewater of beer factory.The results come from fermentation showed that the amount of hydrogen produced, at conditions of initial pH=7.0, glucose concentration 10000 mg·L^-1 and amount of inoculating sludge 2 g MLVSS·L^-1, was less for untreated sludge than pretreated one.For anaerobic granular sludge treated at 95℃, the maximum accumulative hydrogen production in 120 h, 133.47 ml, was obtained, and equal to hydrogen yields of 1.40 mol·mol^-1 glucose, and specific hydrogen production of 15.93 mmol H₂·(g MLVSS)^-1. Although hydrogen production was varied for different pretreatment temperature, butyrate and acetate were dominated in soluble liquid products for all glucose fermentation tests, and their account was about 80% of the total volatile fatty acids and ethanol.

摘要：

为消除发酵生物制氢系统接种污泥中的耗氢菌，加速系统的启动进程并提高产氢效能，以啤酒厂废水处理车间的厌氧颗粒污泥为对象，通过间歇发酵试验，探讨了经65、80、95、110、121℃处理后的污泥的产氢特性。葡萄糖间歇发酵试验证明，在初始pH 7.0、葡萄糖浓度10000 mg·L^-1、污泥接种量2 g MLVSS·L^-1等条件下，由热处理后的活性污泥构建的发酵系统，其产氢量均大于未经处理的活性污泥系统。其中，经95℃处理过的厌氧活性污泥具有更高的发酵产氢性能，在120 h的发酵过程中，其累积产氢量为133.