Abstract:

A hydrocarbon-degrading facultative strain WJ-1 was isolated from the formation water of Menggulin oil reservoir and identified as Pseudomonas aeruginosa by the physiological-biochemical tests and 16S rDNA technology. In order to investigate the biodegradation characteristics of this strain with crude oil as sole carbon source，four different crude oils gathered from Xinjiang，Zhongchakou，Daqing-Jiangqiao，Menggulin oil reservoirs were used. The biodegradation effect on four fractions(saturated hydrocarbons，non-hydrocarbons，aromatics，asphaltene and resin)of oil were analyzed by rod thin layer chromatography and the results showed that the relative contents of non-hydrocarbons，asphaltene and resin decreased in average by above 10% after WJ-1 strain metabolism. Gas chromatography experiments indicated that the light components of saturated hydrocarbons increased，the heavy components of saturated hydrocarbons and phenanthrene of aromatics decreased. The viscosity and solidification point of four crude oils in average reduced by about 45% and 6℃. Physical simulation studies using four different crude oils in porous media showed that the strain WJ-1 could enhance oil recovery by 4.78%，5.15%