›› 2012, Vol. 20 ›› Issue (2): 352-358.

• SELECTED PAPERS FROM THE 6TH WORLD CONGRESS ON INDUSTRIAL PROCESS TOMOGRA-PHY (WCIPT6) • Previous Articles     Next Articles

Tracking submicron particles in microchannel flow by microscopic holography

LUO Rui1, LIU Shi2   

  1. 1 State Key Laboratory of Control and Simulation of Power System and Generation Equipments, Department of Thermal Engineering, Tsinghua University, Beijing 100084, China 2 State Key Laboratory of Alternate Electrical Power System with Renewable Energy Sources, North China Electric Power University, Beijing 102206, China
  • Received:2011-12-02 Online:2012-01-03 Published:2012-04-28

应用显微全息方法对微槽流动中亚微米颗粒的追踪

罗锐1, 刘石2   

  1. 1 State Key Laboratory of Control and Simulation of Power System and Generation Equipments, Department of Thermal Engineering, Tsinghua University, Beijing 100084, China 2 State Key Laboratory of Alternate Electrical Power System with Renewable Energy Sources, North China Electric Power University, Beijing 102206, China

Abstract: Three-dimensional tracking of submicron particles in flows in a micro-channel was carried out using in-line holographic microscopy. A fixed single 0.5 μm fluorescent particle was identified and isolated from dust particles or overlapped particle pair using the laser induced fluorescent (LIF) method. Then in-line microscopic holograms of the fixed single particle were obtained at different positions on the optical axis, i.e. the defocus distances. The holograms of the single particle were used as the model templates with the known defocus distances. The particles in the in-line microscopic holograms of flow in the microchannel were then identified and located to obtain their two-dimensional positions. The defocus distances of those particles were determined by matching each hologram pattern to one of the model templates obtained in the single particle test. Finally the three-dimensional position and velocity of each particle were obtained.

Key words: in-line hologram, microscopic holography, submicron particle, micro-channel laser induced fluorescence

摘要: Three-dimensional tracking of submicron particles in flows in a micro-channel was carried out using in-line holographic microscopy. A fixed single 0.5 μm fluorescent particle was identified and isolated from dust particles or overlapped particle pair using the laser induced fluorescent (LIF) method. Then in-line microscopic holograms of the fixed single particle were obtained at different positions on the optical axis, i.e. the defocus distances. The holograms of the single particle were used as the model templates with the known defocus distances. The particles in the in-line microscopic holograms of flow in the microchannel were then identified and located to obtain their two-dimensional positions. The defocus distances of those particles were determined by matching each hologram pattern to one of the model templates obtained in the single particle test. Finally the three-dimensional position and velocity of each particle were obtained.

关键词: in-line hologram, microscopic holography, submicron particle, micro-channel laser induced fluorescence