Abstract: Partition characteristics of straw hydrolysate in an acetonitrile–aqueous two-phase system were investigated in this paper. The formation of an aqueous two-phase system was investigated in detail. The effects of the volumetric ratio between acetonitrile and straw hydrolyzate，and temperature on the distribution coefficient of sugar were studied，respectively. When the volume fraction of acetonitrile was lower than 50%，an aqueous two-phase system could not form in this study. In addition，it was observed that the glucose，reducing sugar，and polysaccharide existed in the upper phase rich in acetonitrile when the ratio of acetonitrile and straw hydrolyzate was equal to 2 or 3，and the experiments temperature was 25 ℃. Especially，when the ratio of acetonitrile and straw hydrolyzate is equal to 3，the yield of glucose extraction was 81.56% in upper phase. When the phase separation experiments were carried out at 0 ℃，only polysaccharide was detected in the upper acetonitrile phase.

Key words: biomass resources, rice straw, aqueous two-phase system, acetonitrile, sugar

摘要： 研究了稻草水解液中的糖在乙腈-水双水相体系中的分配行为，首先重点考察了双水相的形成，分析了乙腈与稻草水解浓缩液的体积比和温度等对糖的分配系数影响。乙腈体积分数低于50%时不能形成双水相。当V乙腈：V样品等于2或者3时，在25 ℃下实验，上层富含乙腈相中有葡萄糖、还原糖以及多糖同时存在，特别是当V乙腈：V样品等于3时，81.56%的葡萄糖提取到乙腈相中。在0 ℃下实验，上层富含乙腈相中都只检测到多糖。

关键词: 生物质资源, 稻草, 双水相系统, 乙腈, 糖