Abstract: Taking the industrial pleuromutilin fermentation with strain Pleurotus mutilus as an example, the variations of small molecule metabolomes in different industrial fermentation processes were analyzed.The small molecule metabolomes in different processes were measured by gas chromatography coupled to time-of-flight mass spectrometry.Principal component analysis（PCA)was performed on the metabolome of P.mutilus.According to the score plot and loading plot obtained by PCA, the similarities and differences of metabolism of P.mutilus in two processes were extracted and classified.The analysis on characteristic metabolites indicated significant effects of different processes on cell viabilities.The higher level of TCA cycle intermediates and amino acids during the seed culture stage in the process two indicated higher carbon and nitrogen metabolism in P.mutilus before the inoculation.In addition, the analysis of the variations of metabolites during the two different fermentation processes suggested that nutrition utilization efficiency of P.mutilus in process two was higher than that in process one.This study indicates that metabolome analysis could be a new approach to evaluating the industrial bioprocess and is helpful in providing the feasible regulation scheme to improve the fermentation process.

Key words: industrial process, metabolomics, principal components analysis, characteristic metabolites, pleuromutilin

关键词: 工业过程, 代谢组学, 主成分分析, 特征代谢物, 截短侧耳素