Abstract: Wood sawdust of different sizes and cylindrical activated carbon were used as the immobilization carriers of Thiobacillus ferrooxidans，and a fixed-bed bioreactor was constructed. The bio-oxidation of ferrous iron by Thiobacillus ferrooxidans biofilm at different aeration rates and different dilution rates were studied. The result showed that the maximum oxidation was 5.83 gFe2+·L－1·h－1 in the fixed-bed bioreactor of 0.65 L with 12 mm×5 mm×1 mm sized wood sawdust carrier at aeration rates of 1.4 L·min－1 and dilution rate of 1.1 h－1. Immobilization of Thiobacillus ferrooxidans and bio-oxidation of ferrous iron with different carriers were compared. Wood sawdust showed much better efficiency than activated carbon in immobilization and oxidation of ferrous iron.

摘要： 采用不同尺寸的木屑和圆柱状的活性炭作为氧化亚铁硫杆菌的固定化载体，构建了固定床生物反应器。实验考察了间歇操作下的通气速率、连续操作下的稀释率等参数对反应器中氧化亚铁硫杆菌菌膜氧化Fe2+过程的影响。实验结果表明，在0.65 L固定床反应器中，以尺寸为12 mm×5 mm×1 mm的木屑作为固定化载体，控制通气速率为1.4 L/min，稀释率为1.1 h－1时，可获得最大Fe2+平均氧化速率5.83 g/(L·h)。对不同载体的氧化亚铁硫杆菌固定化以及Fe2+氧化情况做出了对比。