Abstract: In order to study the effect of different carbon sources on the phosphorus release,PHA transform,population of the phosphate accumulating organisms(PAOs),and the anaerobic stoichiometry of PHA and glycogen,three enhanced biological phosphorus removal(EBPR)reactors were operated for a long term in the sequencing batch reactor(SBR)fed with acetate/propionate,glucose,or domestic wastewater.The result showed that three systems acquired stable phosphorus removal efficiency from the 182nd day,and FISH results showed that PAOs accounted for 89%?3%,55%?3%,45%?4% of total biomass.Polyhydroxybutyrate and polyhydroxyvalerate were synthesized by PAOs when fed with acetate,glucose,or domestic wastewater as carbon source,only polyhydroxyvalerate was synthesized when fed with propionate as carbon source,and no poly-3-hydroxy-2- methylvalerate(PH2MV)was detected.Stoichiometry research showed that 1.15 C-mol of PHB and 0.15 C-mol of PHV were synthesized per C-mol acetate,meanwhile 0.47 C-mol of glycogen was degraded.0.44 C-mol of PHV was synthesized and 0.271 C-mol of glycogen was degraded when fed with 1 C-mol propionate.0.16 C-mol of PHV was synthesized and 0.16 C-mol of glycogen was degraded when fed with one C-mol glucose,while very few of PHB was synthesized during this process.The proportions of PHB/COD and PHV/COD were 0.98 and 0.13 respectively when fed with domestic wastewater.Release of phosphorus reached the maximum 134 mg稬^-1 and release rate was 23.80 mg P?(g VSS)^-1穐^-1 with acetate as sole carbon source.With propionate or glucose as carbon source,phosphorus release rate was similar,while with domestic wastewater as carbon source phosphorus release rate was the smallest.

Key words: EBPR, FISH, carbon source, PHA, glycogen, stoichiometry

摘要： 采用乙酸/丙酸交替、葡萄糖、实际生活污水为碳源长期驯化的三个强化生物除磷系统,研究了不同碳源对磷的释放和聚羟基烷酸(PHA)转化的影响、聚磷菌种群结构以及微生物代谢PHA和糖原的厌氧化学计量学。结果表明,从182 d起三个系统均获得稳定的除磷性能,第300 d三个系统内聚磷菌所占全菌的比例分别达到:89%±3%、55%±3%、45%±4%。乙酸、葡萄糖、生活污水为碳源时,聚磷菌细胞内贮存聚羟基丁酸(PHB)和聚羟基戊酸(PHV),丙酸为碳源PHA完全由PHV组成,四种类型碳源都未检测到聚二甲基三羟基戊酸(PH2MV)的生成。计量学研究表明:聚磷菌吸收1 C-mol的乙酸,细胞内合成1.15 C-mol PHB,0.15 C-mol PHV,分解0.47 C-mol糖原;吸收1 C-mol的丙酸生成0.44 C-mol的PHV,分解0.271 C-mol的糖原;吸收1C-mol的葡萄糖生成极少量的PHB和0.16C-mol PHV,分解0.16 C-mol糖原;以实际生活污水为碳源,消耗1 mg的COD,合成0.98 mg PHB、0.13 mg PHV(以COD计)。当以乙酸为碳源时获得最高的厌氧释磷量及最大的释磷速率,分别为:134 mg·L^-1和23.80 mg P·(g VSS)^-1·h^-1。以丙酸与葡萄糖为碳源时释磷速率相似,以生活污水为碳源的情况下释磷速率最小。

关键词: EBPR, FISH, 碳源, PHA, 糖原, 化学计量学