Interaction of cellulase with surfactants and their application in detergent

doi:10.11949/j.issn.0438-1157.20151989

Abstract

Abstract:

Effects of surfactants on cellulase activity were studied. The interaction mechanism between surfactants and cellulase was discussed by dynamic light scattering (DLS), fluorescence spectrum and ATR-FTIR. The DLS results showed that anionic surfactants were adsorbed onto cellulase surface, which made cellulase zeta potential distribute in the lower negative charge region. There was no obvious difference of cellulase zeta potential between in nonionic surfactants and deionized water. As shown in spectroscopy, the anionic surfactants had stronger influence on the main chain and side chain of cellulase than nonionic surfactant AEOs. The washing performance data of liquid detergent with and without cellulase indicated that adding cellulase into liquid detergent can enhance detergency of liquid detergent.

Key words: cellulase, surfactant, DLS, fluorescence spectrum, ATR-FTIR, detersive power

摘要：

研究了表面活性剂对纤维素酶活力的影响，并通过动态光散射（DLS）、荧光光谱及傅里叶变换衰减全反射红外光谱（ATR-FTIR）探讨了表面活性剂和纤维素酶的相互作用机理。DLS实验结果显示，阴离子表面活性剂吸附到了纤维素酶表面，使得阴离子表面活性剂-纤维素酶体系中的zeta电位分布在较低的负电荷区域；而非离子表面活性剂-纤维素酶与蒸馏水-纤维素酶体系中的zeta电位的分布没有明显的区别。荧光和ATR-FTIR实验说明了阴离子表面活性剂对纤维素酶的二级结构和侧链的微环境都有很大的影响。此外，对比了添加与未添加纤维素酶的洗衣液对国标污布JB-03的去污力，通过光学显微镜及扫描电镜（SEM）观察国标污布洗涤前后织物表面，发现将纤维素酶添加到洗衣液能明显提高洗衣液的去污能力。

关键词: 纤维素酶, 表面活性剂, 动态光散射, 荧光光谱, ATR-FTIR, 去污力

CLC Number:

TQ028.8

YU Yue, ZHANG Jian. Interaction of cellulase with surfactants and their application in detergent[J]. CIESC Journal, 2016, 67(7): 3023-3031.

于跃, 张剑. 纤维素酶与表面活性剂的相互作用及其在洗涤剂中的应用[J]. 化工学报, 2016, 67(7): 3023-3031.

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