Abstract: In biomedical engineering and micro chemical analysis,the microinjection system based on glass micropipette often have some disadvantages such as seepage or regurgitation during injection because of glass hydrophilicity.It is difficult to detect and control the system and the injection effect from micro pipette is also affected.This paper presents a novel technology of hydropholic treatment.The designated region of inner and outer micropipette surface is treated in a hydrophobic way.The droplet is injected with a spherical shape when liquid flow is driven and controlled digitally,which improves greatly the injection effect,and the amount of injected fluid which may reach an ultra high resolution of the order of femtoliter can be accurately detected and controlled.The validity of the presented method is verified by experiment.

摘要： 在生物医学和微化学分析领域, 基于玻璃微针的微注射系统由于玻璃基质的亲水性，在微注射过程中会出现渗流、回流的现象，影响注射效果，不利于微注射量的检测和控制.本文介绍的疏水化工艺通过将微喷嘴（微针）内外表面特定区域进行疏水化处理，对微流体进行数字化驱动和控制,可改善微流体流动特性，提高微注射效果，使液滴按一定规律以球形颗粒喷射，有助于微流量的精确检测和微喷射的精确量可控.