Abstract:

The determination of isotherm and prediction of retention values are the basis of the optimization of separation conditions of preparative chromatography.In this paper, the isotherms of ginsenoside Rg₁ and Re from Panax ginseng were studied.Ginsenoside Rg₁ and Re were prepared by chromatography column from Panax ginseng raw material, and the isotherms of ginsenoside Rg₁ and Re were determined by frontal analysis (FA) on spherical C₁₈ solid phase.The adsorption parameters G and b were set based on the competitive Langmuir model .In the mean time, the retention time of ginsenoside Re was obtained with the results of nonlinear chromatography theory.The calculated retention time were compared with that of experimental results with average relative error 3.68%.Additionally, some problems related to fitting based on the competitive Langmuir model were found and discussed.

Key words:

吸附等温线, 迎头法, 非线性色谱, 双组分, 竞争Langmuir, 人参皂苷Rg₁, 人参皂苷Re

摘要： 制备色谱分离条件优化的基础是吸附等温线的测定及保留时间的预测。本文以人参皂甙Rg₁、Re为研究对象，用色谱单柱制备了人参粗粉中的人参皂甙Rg₁、Re,以迎头法测定了人参皂甙Rg₁、Re在C₁₈球形固定相中的吸附等温线，在竞争Langmuir模型基础上确定了吸附参数G和b，并应用非线性色谱理论的结果预测了人参皂甙Re的保留时间，上述结果与实验值进行了比较，平均相对误差为3.68%。在实验中发现了运用竞争Langmuir模型进行拟合的一些问题并进行了讨论。

关键词:

吸附等温线, 迎头法, 非线性色谱, 双组分, 竞争Langmuir, 人参皂苷Rg₁, 人参皂苷Re