Abstract:

The optimum conditions and effectiveness of extraction of extracellular polymeric substances (EPS) from activated sludge in a membrane bioreactor（MBR） were studied under such conditions, thermal, acid, alkaline treatment methods, and it was concluded that both thermal treatment (80℃，45 min) and EDTA bi-sodium treatment were effective.The sludge cell was damaged seriously by NaOH treatment (1 mol·L^-1,2 h), but when formaldehyde was added, the nucleic acid concentration was reduced by 21.5%, and the damage to sludge cell was then decreased.

Key words:

膜生物反应器, 胞外聚合物, EDTA二钠, 热提取, NaOH提取, 甲醛

摘要： 采用七种方法（热、酸、碱处理等）提取MBR（Membrane Bioreactor）中污泥EPS（extracellular polymeric substances），研究了这些方法对于污泥EPS的最佳提取条件。在综合考虑各种方法的精确性、操作简易度、提取效果、对污泥细胞的破坏程度后，确定EDTA二钠提取（浓度2%，3h）和热提取（80℃，45min）是最简便有效的两种提取方法。NaOH提取（1mol.L^-1，2h）对污泥细胞具有很大的破坏作用，投加甲醛（浓度2%，2h）之后提取核酸含量减少了21.5%，降低了NaOH溶液提取对于细胞的破坏作用。

关键词:

膜生物反应器, 胞外聚合物, EDTA二钠, 热提取, NaOH提取, 甲醛