Abstract: Substrate limited fed batch cultures were used to study the growth, metabolism and antibody production of HB 58 hybridoma cells. Glucose or glutamine limitation decreased specific growth rate. In the condition of glucose limitation, lactate formation and Y_Lac/Glc decreased, but Y_cell/Glc increased, which suggested that more glucose took part in TCA cycle. In the condition of glutamine limitation, the formation of ammonia and alanine and Y_Ala/Gln decreased, but Y_cell/Gln and Y_Amm/Gln increased, which indicated that more glutamine was incorporated into dehydrogenation reaction and its effective utilization was achieved.

Key words: 杂交瘤细胞, 流加培养, 葡萄糖限制, 谷氨酰胺限制

摘要： 对底物限制的流加培养中杂交瘤细胞HB 58的生长、代谢和单抗生成进行了研究。葡萄糖或
谷氨酰胺限制引起比生长速率下降。葡萄糖限制时，乳酸生成减少，细胞对葡萄糖的得率增
加，乳酸对葡萄糖的得率降低，说明葡萄糖更多地参与三羧酸循环。谷氨酰胺限制时，氨和丙氨酸生成减少，细胞和氨对谷氨酰胺的得率增加，丙氨酸对谷氨酰胺的得率减小，说明谷氨酰胺更多地参与脱氢反应，其有效利用率得到提高。

关键词: 杂交瘤细胞, 流加培养, 葡萄糖限制, 谷氨酰胺限制