Abstract:

In order to separate amino acids whose isoelectric points (IP) are near to each other (taking proline and serine as example), a multistage electrodialysis process was developed.In the experiment, the influences of number of stages, current intensity and concentration of NaOH on separation efficiency were investigated.The results showed that it was feasible to separate near IP amino acids by using multistage electrodialysis.Increasing the number of stages could obviously improve the purity of proline product, while have little effect on the purity of serine product，increasing current intensity could improve the purity of proline product, while have less effect on the purity of serine product.A proper amount of NaOH in the feed was helpful to the separation.

Key words:

多级电渗析, 丝氨酸, 脯氨酸, 阴离子交换膜, 双极性膜

摘要： 为解决等电点相近的氨基酸的分离问题，采用多级连续电渗析，以丝氨酸（Ser）和脯氨酸（Pro）为例，探讨了级数、电流和NaOH浓度对分离性能的影响。实验结果表明，用多级连续电渗析分离近等电点氨基酸是可行的。增加级数，可提高脯氨酸产品纯度，但丝氨酸产品纯度变化不大。增大电流，脯氨酸产品纯度有所提高，而丝氨酸产品纯度略有下降。在原料中加入适量的NaOH有利于提高分离性能。

关键词:

多级电渗析, 丝氨酸, 脯氨酸, 阴离子交换膜, 双极性膜