不同C/N下SBBR脱氮过程N2O释放及胞外多聚物变化

doi:10.11949/0438-1157.20190753

摘要/Abstract

摘要：

在(20±2.0)℃条件下，利用序批式生物膜反应器（sequencing batch biofilm reactor，SBBR），考察不同碳氮比（C/N=3.0、5.0、8.0和10.0）下同步脱氮（simultaneous nitrification and denitrification，SND）过程N₂O释放及胞外聚合物（extracellular polymeric substance，EPS）变化。C/N由3.0增至10.0，异养菌大量增殖，曝气阶段DO降低，系统硝化性能受到抑制，SBBR系统出水NH₄⁺由0.5 mg/L以下增至（7.85±1.42）mg/L，N₂O产量由（2.68±0.17）mg/L降至（1.02±0.12）mg/L。C/N=8.0，TN去除率最大为80.4%±3.5%。反应初期，微生物体内聚β-羟基烷酸酯（PHA）增加，可为后续反硝化过程提供电子供体。AOB好氧反硝化和低氧条件下异养菌反硝化过程均可导致N₂O产生。C/N降低，SBBR内部缺氧区域减少，N₂O还原过程减弱，释放量增加；C/N增加，N₂O扩散进入生物膜内缺氧区域，促进其减量。C/N由3.0增至10.0，微生物EPS分泌由（57.6±5.6）mg / g VSS降至（32.7±3.2）mg / g VSS，其中，TB-EPS含量占65.8%~68.8%。低C/N下，紧密型EPS（TB-EPS）中多糖（PS）含量增加，生物膜更加密实，N₂O扩散进入缺氧区阻力增加，释放量增加。

关键词: C/N, 同步硝化反硝化, N₂O, 胞外多聚物

Abstract:

At normal temperature of (20±2.0)℃, the characteristics of N₂O eimssion and extracellular polymeric substance(EPS) production were studied during simultaneous nitrification and denitrification(SND) process under different C/N ratio (C/N=3.0,5.0, 8.0 and 10.0). When C/N increased from 3.0 to 10.0, the heterotrophic bacteria increased rapidly, DO decreased and the nitrification process was inhibited. The effluent NH₄⁺ increased from below 0.5 mg/L to (7.85±1.42) mg/L, and the N₂O emission decreased from (2.68±0.17) mg/L to (1.02±0.12) mg/L. When C/N was 8.0, the maximum total notrogen removal efficiency was 80.4%±3.5%. The accumulation of PHA(poly-β-hydroxyalkanoate) increased firstly and then decreased. Both nitrifier denitrification and heterotrophic denitrification resulted in N₂O production. The anoxic area located in the biofilm decreased with the decreasing of C/N ratio, which weakened the denitrification process. With the increase of C/N ratio, N₂O diffused into the anoxic region of biofilm can be effectively reduced. The microbial EPS secretion decreased from (57.6±5.6) mg / g VSS to (32.7±3.2) mg / g VSS. The TB-EPS content accounts for 65.8%—68.8% of the total EPS. Under low C/N, the content of polysaccharide (PS) in compact EPS (TB-EPS) increased, the biofilm was more compact, the resistance of N₂O diffusion into the anoxic zone increased, and the release increased.

Key words: C/N ratio, simultaneous nitrification and denitrification, N₂O, extracellular polymeric substance

中图分类号:

X 703.1

巩有奎, 赵强, 彭永臻. 不同C/N下SBBR脱氮过程N₂O释放及胞外多聚物变化[J]. 化工学报, 2019, 70(12): 4847-4855.

Youkui GONG, Qiang ZHAO, Yongzhen PENG. Variation of N₂O emission and EPS production during simultaneous nitrification and denitrification in SBBR under different C/N ratio[J]. CIESC Journal, 2019, 70(12): 4847-4855.

图/表 6

图1 试验装置图

Fig.1 Schematic diagram of SBBR system

图2 不同 C/N下SBBR系统污染物变化及脱氮效率

Fig.2 Performance of nitrogen removal in SBBR under different C/N ratio during the whole process

图3 不同DO下SBBR系统内部微生物SOUR

Fig.3 Effect of C/N ratio of influent on specific oxygen uptake rate (SOUR) in SBBR

图4 不同C/N下SBBR典型周期COD、氮素和PHA变化

Fig.4 Variation of COD, nitrogen and PHA concentrations in typical operational cycles of SBBR under different C/N ratio

图5 不同进水C/N下SBBR系统EPS、LB-EPS和TB-EPS含量变化

Fig.5 Effect of C/N ratio of influent on total amount of EPS, LB-EPS and TB-EPS in SBBR

图6 不同进水C/N下TB-EPS (a)和LB-EPS (b)中PN、PS变化

Fig.6 Effect of C/N ratio of influent on PN and PS amount in TB-EPS (a) and LB-EPS (b)

参考文献 36

1	Wang J, Rong H, Zhang C. Evaluation of the impact of dissolved oxygen concentration on biofilm microbial community in sequencing batch biofilm reactor[J]. Journal of Bioscience and Bioengineering, 2018, 125(5): 532-542.
2	Zhao J, Feng L, Yang G, et al. Development of simultaneous nitrification-denitrification (SND) in biofilm reactors with partially coupled a novel biodegradable carrier for nitrogen-rich water purification[J]. Bioresource Technology, 2017, 243: 800-809.
3	Sabba F, Terada A, Wells G, et al. Nitrous oxide emissions from biofilm processes for wastewater treatment[J].Applied Microbiology and Biotechnology, 2018, 102(22): 9815-9829.
4	Mahne I, Princic A, Megusar F. Nitrification/denitrification in nitrogen high-strength liquid wastes[J].Water Research, 1996, 30(9): 2107-2111.
5	Her J J, Huang J S. Influences of carbon source and C/N ratio on nitrate/nitrite denitrification and carbon breakthrough [J]. Bioresource Technology, 1995, 54(1): 45-51.
6	Wang X, Chen Z, Shen J, et al. Impact of carbon to nitrogen ratio on the performance of aerobic granular reactor and microbial population dynamics during aerobic sludge granulation[J]. Bioresource Technology, 2019, 271: 258-265.
7	Chao C, Zhao Y, Keskar J, et al. Simultaneous removal of COD, nitrogen and phosphorus and the tridimensional microbial response in a sequencing batch biofilm reactor: with varying C/N/P ratios[J/OL].Biochemical Engineering Journal, https: //doi.org/10.1016/j.bej.2019.04.017.
8	Ye F X, Ye Y F, Li Y. Effect of C/N ratio on extracellular polymeric substances (EPS) and physicochemical properties of activated sludge flocs [J]. Journal of Hazardous Materials, 2011, 188(1/2/3): 37-43.
9	Wang Z, Gao M, Xin Y, et al. Effect of C/N ratio on extracellular polymeric substances of activated sludge from an anoxic-aerobic sequencing batch reactor treating saline wastewater [J]. Environmental Technology, 2014, 35(24): 2821-2828.
10	Ravishankara A R, Daniel J S, Portmann R W. Nitrous oxide(N₂O): the dominant ozone-depleting substance emitted in the 21st century [J]. Science, 2009, 326(5949): 123-125.
11	Ye L, Ni B J, Law Y Y, et al. A novel methodology to quantify nitrous oxide emissions from full-scale wastewater treatment systems with surface aerators[J]. Water Research, 2014, 48(1): 257-268.
12	Stefano M, Giaime T, Giovannimatteo E. Evaluation of nitrous oxide gaseous emissions from a partial nitritation reactor operating under different conditions[J]. Desalination and Water Treatment, 2018, 127: 158-164.
13	Kong Q, Wang Z, Niu P, et al. Greenhouse gas emission and microbial community dynamics during simultaneous nitrification and denitrification process [J]. Bioresource Technology, 2016, 210: 94-100.
14	Jia W, Liang S, Zhang J, et al. Nitrous oxide emission in low-oxygen simultaneous nitrification and denitrification process: sources and mechanisms [J]. Bioresource Technology, 2013, 136: 444-451.
15	APHA(American Public Health Association). Standard methods for the examination of water and wastewater[S]. Baltimore: Port City Press, 1998.
16	Oehmen A, Keller-Lehmann B, Zeng R J, et al. Optimisation of poly-beta-hydroxyalkanoate analysis using gas chromatography for enhanced biological phosphorus removal systems [J]. Journal of Chromatography A, 2005, 1070(1/2): 131-136.
17	Yang Q, Liu X H, Peng C Y, et al. N₂O production during nitrogen removal via nitrite from domestic wastewater: main sources and control method[J]. Environmental Science & Technology, 2009, 43(24): 9400-9406.
18	张建华, 彭永臻, 张淼, 等. 同步硝化反硝化SBBR处理低C/N比生活污水的启动与稳定运行 [J]. 化工学报, 2016, 67(11): 4817-4824.
	Zhang J H, Peng Y Z, Zhang M, et al. Start-up and steady operation of simultaneous nitrification and denitrification in SBBR treating low C/N ratio domestic wastewater[J]. CIESC Journal, 2016, 67(11): 4817-4824.
19	巩有奎, 任丽芳, 彭永臻. 不同DO下SBBR亚硝酸型同步脱氮及N₂O释放特性 [J]. 化工学报, 2019, 70(4): 1550-1558.
	Gong Y K, Ren L F, Peng Y Z. Characteristics of simultaneous nitrification and denitrification via nitrite and N₂O emission in SBBR under different DO concentrations[J]. CIESC Journal, 2019, 70(4): 1550-1558.
20	王荣昌, 肖帆, 赵建夫. 生物膜厚度对膜曝气生物膜反应器硝化性能的影响 [J]. 高校化学工程学报, 2015, 29(1): 151-158.
	Wang R C, Xiao F, Zhao J F. Effects of biofilm thickness on nitrification performance of membrane-aerated biofilm reactors[J]. Journal of Chemical Engineering of Chinese Universities, 2015, 29(1): 151-158.
21	Bradford M M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding [J]. Analytical Biochemistry, 1976, 72(1): 248-254.
22	Dubois M, Gilles K A, Hamilton J K, et al. Colorimetric method for determination of sugars and related substances [J]. Analytical Chemistry, 1956, 28(3): 350-356.
23	戴娴, 王晓霞, 彭永臻, 等. 进水C /N 对富集聚磷菌的SNDPR系统脱氮除磷的影响[J]. 中国环境科学, 2015, 35(9): 2636-2643.
	Dai X, Wang X X, Peng Y Z, et al. Effect of influent C/N ratio on simultaneous nitrification-denitrification and phosphorus removal (SNDPR) enriched with phosphorus accumulating organisms (PAOs)[J]. China Environmental Science, 2015, 35(9): 2636-2643.
24	Nogueira R, Melo L F, Purkhold U, et al. Nitrifying and heterotrophic population dynamics in biofilm reactors: effects of hydraulic retention time and the presence of organic carbon[J]. Water Research, 2002, 36(2): 469-481.
25	赵青, 卞伟, 李军, 等. DO/NH₄⁺-N实现短程硝化过程中生物膜特性[J]. 环境科学, 2018, 39(3): 1278-1285.
	Zhao Q, Bian W, Li J, et al. Characteristics of biofilm during the transition process of complete nitrification and partial nitrification [J].Environmental Science, 2018, 39(3): 1278-1285.
26	Yang S, Yang F L, Fu Z M, et al. Simultaneous nitrogen and phosphorus removal by a novel sequencing batch moving bed membrane bioreactor for wastewater treatment[J]. Journal of Hazardous Materials, 2010, 175(1/2/3): 551-557.
27	Peng L, Ni B J, Erler D, et al. The effect of dissolved oxygen on N₂O production by ammonia-oxidizing bacteria in an enriched nitrifying sludge[J]. Water Research, 2014, 66(1): 12-21.
28	Gong Y, Peng Y, Yang Q, et al. Formation of nitrous oxide in a gradient of oxygenation and nitrogen loading rate during denitrification of nitrite and nitrate[J]. Journal of Hazardous Materials, 2012, 227/228: 453-460.
29	Zeng R J, Romain L, Yuan Z, et al. Simultaneous nitrification, denitrification, and phosphorus removal in a lab-scale sequencing batch reactor[J]. Biotechnology & Bioengineering, 2003, 84(2): 170-178.
30	曾薇, 张悦, 李磊. 限氧曝气实现亚硝酸型同步硝化反硝化的研究[J]. 北京工业大学学报, 2010, 36(9): 1263-1270.
	Zeng W, Zhang Y, Li L.Simultaneous nitrification denitrification via nitrite under limited aeration[J]. Journal of Beijing University of Technology, 2010, 36(9): 1263-1270.
31	Letey J, Valoras N, Focht D D, et al. Nitrous oxide production and reduction during denitrification as affected by redox potential[J].Soil Science Society of America Journal, 1981, 45(4): 727-730.
32	刘越, 李鹏章, 彭永臻, 等. 短程硝化过程中硝化速率与N₂O产生速率的关系[J].化工学报, 2015, 66(11): 4652-4660.
	Liu Y, Li P Z, Peng Y Z, et al. Relationship between N₂O production rate and ammonia oxidation rate during nitritation process[J]. CIESC Journal, 2015, 66(11): 4652-4660.
33	Chen X, Yuan Z, Ni B J. Nitrite accumulation inside sludge flocs significantly influencing nitrous oxide production by ammonium-oxidizing bacteria[J]. Water Research, 2018, 143(15): 99-108.
34	Rusanowska P, Cydzik-Kwiatkowska A, Świątczak P, et al. Changes in extracellular polymeric substances (EPS) content and composition in aerobic granule size-fractions during reactor cycles at different organic loads[J]. Bioresource Technology, 2019, 272: 188-193.
35	Kang J A, Yuan Q. Long-term stability and nutrient removal efficiency of aerobic granules at low organics loads[J]. Bioresource Technology, 2017, 234: 336-342.
36	孙洪伟, 陈翠忠, 高宇学, 等. 碳氮比对活性污泥胞外聚合物的长期影响[J].中国环境科学, 2018, 38(3): 950-958.
	Sun H W, Chen C Z, Gao Y X, et al. Effect of C/N ratio on extracellular polymeric substance (EPS) in the sequencing batch reactor (SBR)[J]. China Environmental Science, 2018, 38(3): 950-958.

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不同C/N下SBBR脱氮过程N₂O释放及胞外多聚物变化

Variation of N₂O emission and EPS production during simultaneous nitrification and denitrification in SBBR under different C/N ratio

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