2-KGA混菌发酵大小菌耦合关系研究及建模

doi:10.3969/j.issn.0438-1157.2013.07.029

化工学报 ›› 2013, Vol. 64 ›› Issue (7): 2520-2525.DOI: 10.3969/j.issn.0438-1157.2013.07.029

2-KGA混菌发酵大小菌耦合关系研究及建模

丰强强¹, 潘玉霖¹, 成宝琨¹, 孙君伟², 袁景淇¹

1. 上海交通大学自动化系, 系统控制与信息处理教育部重点实验室, 上海 200240;
2. 河北维尔康制药有限公司, 河北石家庄 050031

收稿日期:2012-12-09 修回日期:2013-01-30 出版日期:2013-07-05 发布日期:2013-07-05
通讯作者: 袁景淇
作者简介:丰强强(1987- ),男,硕士研究生。
基金资助:
博士学科点专项科研基金项目(20110073110018);国家自然科学基金项目(60974068)。

Modeling on interaction of different strains in 2-KGA mixed culture fermentation

FENG Qiangqiang¹, PAN Yulin¹, CHENG Baokun¹, SUN Junwei², YUAN Jingqi¹

1. Department of Automation, Shanghai Jiao Tong University, Key Laboratory of System Control and Information Processing, Ministry of Education, Shanghai 200240, China;
2. Hebei Welcome Pharmaceutical Co., Ltd., Shijiazhuang 050031, Hebei, China

Received:2012-12-09 Revised:2013-01-30 Online:2013-07-05 Published:2013-07-05
Supported by:
supported by the Research Fund for the Doctoral Program of Higher Education (20110073110018)and the National Natural Science Foundation of China (60974068).

摘要/Abstract

摘要： 研究了维生素C前体2-酮基-L-古龙酸(2-KGA)混菌发酵巨大芽孢杆菌(俗称大菌)与普通生酮古龙酸菌(俗称小菌)的耦合关系。经研究发现:(1)大菌通过群体感应调整自身生长行为;(2)大菌活菌代谢物及其自溶物质弥补小菌代谢缺陷促进小菌生长;(3)小菌可能通过释放溶菌酶促使大菌自溶;(4)大菌自溶释放一些蛋白酶类物质提高小菌山梨糖脱氢酶(SDH)活性从而加速产物形成。基于以上研究建立了2-KGA混菌发酵的动力学模型,并用实验数据证明了模型的有效性。

关键词: 2-KGA, 混菌发酵, 群体感应, 信号耦合, 代谢耦合, 动力学模型

Abstract: The interaction between Bacillus megaterium(also called big bacterium)and Ketogulonicigenium vulgare (also called small bacterium)was studied for 2-keto-L-gulonic acid (2-KGA)mixed culture fermentation.The following mechanisms were found.(1)Big bacterium might adjust its growth behavior by quorum sensing.(2)Metabolites and autolysis substances of big bacterium were beneficial to overcoming the metabolic defects of small bacterium and therefore accelerating the latter's growth.(3)Small bacterium released lysozyme to promote big bacterium's autolysis.(4)Big bacterium autolysis released specific protease substances to enhance sorbitol dehydrogenase (SDH)which might increase the synthesis rate of 2-KGA.Based on such mechanisms,a kinetic model of 2-KGA mixed fermentation was established.Model validation was carried out with four sets of experimental data under different cultivation conditions.The results demonstrated that the proposed model was able to well describe the growth of two bacteria and 2-KGA production.

Key words: 2-KGA, mixed culture, quorum sensing, signal coupling, metabolic coupling, dynamic model

中图分类号:

TP273

丰强强, 潘玉霖, 成宝琨, 孙君伟, 袁景淇. 2-KGA混菌发酵大小菌耦合关系研究及建模[J]. 化工学报, 2013, 64(7): 2520-2525.

FENG Qiangqiang, PAN Yulin, CHENG Baokun, SUN Junwei, YUAN Jingqi. Modeling on interaction of different strains in 2-KGA mixed culture fermentation[J]. CIESC Journal, 2013, 64(7): 2520-2525.

参考文献 20

[1]	Yin Guanglin(尹光琳),Tao Zengxin(陶增鑫),Yu Longhua(于龙华),Wang Dasi(王大耜),Dan Jialin(谈家林),Yan Zizheng(严自正),Ning Wenzhu(宁文珠),Wang Changhui(王长会),Wang Shuding(王书鼎),Jiang Huifeng(姜慧凤),Zhang Xiuming(张秀明),Feng Xiaoyun(冯晓云),Zhao Qiang(赵强),Wei Wenqiao(魏文巧).Studies on the production of vitamin C precursor:2-keto-L-gulonic acid from L-sorbose by fermentation(Ⅰ):Isolation,screening and identification of 2-keto-L-gulonic acid production bacteria[J].Acta Microbiological Sinica (微生物学报),1980,20:246-251
[2]	Han Xiaodong(韩晓东),Zhang Weicai(张惟材),Zhang Tong(张通).Progress in vitamin C fermentation[J].Letters in Biotechnology (生物技术通讯),2009,20(3):433-435
[3]	Wei Dongzhi(魏东芝),Yuan Weikang(袁渭康),Yin Guanglin(尹光琳),Yuan Zhongyi(袁中一),Chen Minheng(陈敏恒).Study on kinetic model of vitamin C two-step fermentation process[J].Chinese Journal of Biotechnology (生物工程学报),1992,8:277-282
[4]	Li Qiang(李强),Cao Zhu'an(曹竹安).Kinetics study of producing 2-keto-L-gulonic acid in mixed fermentation(Ⅱ):Analyze fermentation process by kinetic model//Chemical Industry and Engineering Society of China.Proceedings of the 9th National Conference of Biochemical Engineering (中国化工学会第九届全国生物化工学术会议论文集)[C].Beijing:Chemical Industry Press,2000:167-171
[5]	Zhong Chongbin (仲崇斌),Zhang Zhongze (张忠泽),Zhang Wenge(张文革).Effect of physiologic condition of two strains on production of vitamin precusor 2-keto-L-gulonic acid[J].Biotechnology (生物技术),2003,13(2):34-35
[6]	Lü Shuxia(吕淑霞),Feng Shu(冯树),Zhang Zhongze(张忠泽),Liu Yang(刘阳),Xie Zhanwu(谢占武),An Haiying(安海英).The effect of Bacillus megaterium in vitamin C two-step fermentation[J].Microbiology China (微生物学通报),2001,5:10-13
[7]	Liu Liming,Chen Kejie,Zhang Jing,Liu Jie,Chen Jian.Gelatin enhances 2-keto-L-gulonic acid production based on Ketogulonigenium vulgare genome annotation[J].Journal of Biotechnology,2011,156:182-187
[8]	Gamer M,Stammen S.Bacillus megaterium—an alternative expression system[J].Journal of Biotechnology,2007,131:220
[9]	Chen Kejie(陈克杰),Zhou Jingwen(周景文),Liu Liming(刘立明),Liu Jie(刘杰),Du Guocheng(堵国成),Chen Jian(陈坚).Enhancing 2-keto-L-gulonic acid production under hyperosmotic stress by adding sucrose[J].Chinese Journal of Biotechnology (生物工程学报),2010,26:1507-1513
[10]	Waters C M,Bassler B L.Quorum sensing:cell to cell communication in bacteria[J].Annual Review of Cell and Developmental Biology,2005,21:319-346
[11]	Li Guocai(李国才),Zhang Zhongze(张忠泽).Effects of some additives on fermentation of 2-keto-L-gulonic acid[J].Journal of Microbiology (微生物学杂志),1997,17:10-12
[12]	Zhou Jian,Ma Qian,Song Hai,Wang Lili,Yi Hong,Yuan Yingjin.Metabolome profiling reveals metabolic cooperation between Bacillus megaterium and Ketogulonicigenium vulgare during induced swarm motility[J].Applied and Environmental Microbiology,2011,77:7023-7030
[13]	Murphey E D,Sherwood E R.Pretreatment with the gram-positive bacterial cell wall molecule peptidoglycan improves bacterial clearance and decreases inflammation and mortality in mice challenged with Pseudomonas aeruginosa[J].Microbes and Infection,2008,10:1244-1250
[14]	Zhang Jing,Liu Jie,Shi Zhongping,Liu Liming,Chen Jian.Manipulation of B.megaterium growth for efficient 2-KLG production by K.vulgare[J].Process Biochemistry,2010,45:602-606
[15]	Hoshino T,Sugisawa T,Tazoe M,Shinjoh M,Fujiwara A.Metabolic pathway for 2-keto-L-gulonic acid formation in Gluconobacter melanogenus IFO3293[J].Agricultural and Biological Chemistry,1990,54:1211-1218
[16]	Hanke T.Studies on central carbon metabolism and respiration of Gluconobacter oxydans 621H. Düsseldorf:Heinrich-Heine-Universitt,2009
[17]	Lu Shujuan,Wang Jun,Yao Jianming,Yu Zengliang. Study on the effect of mutated Bacillus megaterium in two-stage fermentation of vitamin C[J].Plasma Science and Technology,2003,5:2011-2016
[18]	Wei Hongli(韦泓丽),Xiong Xianghua(熊向华),Wang Jianhua(汪建华),You Song(游松),Zhang Weicai(张惟材).Impact of the signal peptide of L-sorbose dehydrogenase of Ketogulonigenium vulgare on its expression[J].Letters in Biotechnology (生物技术通讯),2010,5:623-627
[19]	Zhang Zhixiong,Zhu Xinjie,Xie Ping,Sun Junwei,Yuan Jingqi.Macrokinetic model for Gluconobacter oxydans in 2-keto-L-gulonic acid mixed culture[J].Biotechnology and Bioprocess Engineering,2012,17:1008-1017
[20]	Fang Qiang(方强),Chen Dezhao(陈德钊),Yu Huanjun(俞欢军),Wu Xiaohua(吴晓华).Differential evolution algorithm based on eugenic strategy and its application to chemical engineering[J].Journal of Chemical Industry and Engineering(China)(化工学报),2004,55(4):598-602

2-KGA混菌发酵大小菌耦合关系研究及建模

Modeling on interaction of different strains in 2-KGA mixed culture fermentation

PDF (PC)

可视化

被引次数

摘要/Abstract

引用本文

使用本文

参考文献 20

相关文章 15

编辑推荐

Metrics

本文评价

[1]	金正浩, 封立杰, 李舒宏. 氨水溶液交叉型再吸收式热泵的能量及分析[J]. 化工学报, 2023, 74(S1): 53-63.
[2]	李锦潼, 邱顺, 孙文寿. 煤浆法烟气脱硫中草酸和紫外线强化煤砷浸出过程[J]. 化工学报, 2023, 74(8): 3522-3532.
[3]	李艳辉, 丁邵明, 白周央, 张一楠, 于智红, 邢利梅, 高鹏飞, 王永贞. 非常规服役超临界锅炉的微纳尺度腐蚀动力学模型建立及应用[J]. 化工学报, 2023, 74(6): 2436-2446.
[4]	王承泽, 顾凯丽, 张晋华, 石建轩, 刘艺娓, 李锦祥. 硫化协同老化零价铁增效去除水中Cr（Ⅵ）的作用机制[J]. 化工学报, 2023, 74(5): 2197-2206.
[5]	郑杰元, 张先伟, 万金涛, 范宏. 丁香酚环氧有机硅树脂的制备及其固化动力学研究[J]. 化工学报, 2023, 74(2): 924-932.
[6]	周桓, 张梦丽, 郝晴, 吴思, 李杰, 徐存兵. 硫酸镁型光卤石转化钾盐镁矾的过程机制与动态规律[J]. 化工学报, 2022, 73(9): 3841-3850.
[7]	马语峻, 刘向军. 多孔陶瓷膜烟气水分回收理论与模型研究[J]. 化工学报, 2022, 73(9): 4103-4112.
[8]	宋谦石, 王潇伟, 张威, 汪小憨, 李浩文, 乔瑜. 无机元素对生物质焦炭-CO₂气化反应性的催化/抑制作用研究及模型构建[J]. 化工学报, 2022, 73(11): 5240-5250.
[9]	张牧星, 张小松, 丁烨, 宋翼. 氧化石墨烯膜间距对电渗析空气除湿特性影响的分子动力学研究[J]. 化工学报, 2021, 72(S1): 63-69.
[10]	张梦飞, 张玲, 李晓闯, 祖韵秋, 黄明, 石宪章, 刘春太. 厚壁橡胶制品非等温硫化过程模拟与实验研究[J]. 化工学报, 2021, 72(4): 2065-2075.
[11]	李梦钖, 高明, 左启蓉, 章立新, 赵玉刚. 过冷壁面液滴中四丁基溴化铵水合物生成的可视化研究[J]. 化工学报, 2021, 72(4): 2094-2101.
[12]	李超凡, 温玉娟, 曹楠, 孙东, 宋晓明, 杨悦锁. 耐低温对硝基苯酚降解菌的降解动力学研究[J]. 化工学报, 2021, 72(3): 1692-1701.
[13]	杨诗, 蔡阳, 李长平, 李雪辉. 磷钨酸负载锆基金属有机骨架PTA@MOF-808的制备及其吸附脱硫性能[J]. 化工学报, 2021, 72(3): 1722-1731.
[14]	毛桃嫣, 邹敏婷, 郑成, 曾昭文, 伍旭贤, 肖润辉, 彭思玉. 微波化学反应的无量纲准数动力学模型研究：以偶氮二异丁脒盐酸盐（AIBA）分解反应为例[J]. 化工学报, 2021, 72(3): 1364-1371.
[15]	王曰杰, 李玲玲, 何春宏. 炼油废催化剂生物淋滤脱金属研究进展[J]. 化工学报, 2021, 72(2): 901-912.