Abstract: By suspension cultivating of Lithospermum erythrorhizon and determining the dynamic curves, a structural kinetic model was established. By using the equation sets including 14 parameters and 9 state variables,the changes of excellular carbon source, intracellular carbon source, intracellular structural components,intracellular intermediate,cell respiration loss and intra/extracellular products were discussed. The model parameters were estimated by the Runge-Kutta method and the nonlinear Powell method. The sensitivity analysis was done. The simulation results could fit the real curves quite well. It was the first time to use a structural model to describe the suspension culture dynamics of Lithospermum erythrorhizon cells. The structural model was also the foundation for further study.