化工学报 ›› 2008, Vol. 59 ›› Issue (6): 1531-1534.

• 能源和环境工程 • 上一篇    下一篇

膜生物反应器中污泥EPS的提取方法

张宏伟;雷鸣;李莹;张雪花;王捷   

  1. 天津大学环境科学与工程学院
  • 出版日期:2008-06-05 发布日期:2008-06-05

Extraction of extracellular polymeric substances from activated sludge in membrane bioreactor

ZHANG Hongwei;LEI Ming;LI Ying;ZHANG Xuehua;WANG Jie   

  • Online:2008-06-05 Published:2008-06-05

摘要: 采用七种方法(热、酸、碱处理等)提取MBR(Membrane Bioreactor)中污泥EPS(extracellular polymeric substances),研究了这些方法对于污泥EPS的最佳提取条件。在综合考虑各种方法的精确性、操作简易度、提取效果、对污泥细胞的破坏程度后,确定EDTA二钠提取(浓度2%,3h)和热提取(80℃,45min)是最简便有效的两种提取方法。NaOH提取(1mol.L-1,2h)对污泥细胞具有很大的破坏作用,投加甲醛(浓度2%,2h)之后提取核酸含量减少了21.5%,降低了NaOH溶液提取对于细胞的破坏作用。

关键词:

膜生物反应器, 胞外聚合物, EDTA二钠, 热提取, NaOH提取, 甲醛

Abstract:

The optimum conditions and effectiveness of extraction of extracellular polymeric substances (EPS) from activated sludge in a membrane bioreactor(MBR) were studied under such conditions, thermal, acid, alkaline treatment methods, and it was concluded that both thermal treatment (80℃,45 min) and EDTA bi-sodium treatment were effective.The sludge cell was damaged seriously by NaOH treatment (1 mol·L-1,2 h), but when formaldehyde was added, the nucleic acid concentration was reduced by 21.5%, and the damage to sludge cell was then decreased.

Key words:

膜生物反应器, 胞外聚合物, EDTA二钠, 热提取, NaOH提取, 甲醛