CIESC Journal ›› 2010, Vol. 18 ›› Issue (3): 486-492.

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Biodegradation Kinetics of DDT in Soil under Different Environmental Conditions by Laccase Extract from White Rot Fungi

赵月春1, 易筱筠2, 李明华1, 刘露1, 马伟娟1   

  1. 1. Department of Applied Chemistry, College of Science, South China Agricultural University, Guangzhou 510642, China;
    2. College of Environmental Science and Engineering, South China University of Science&Technology, Guangzhou 510640, China
  • 收稿日期:2010-01-16 修回日期:2010-03-29 出版日期:2010-06-28 发布日期:2010-06-28
  • 通讯作者: ZHAO Yuechun,E-mail:yczhao@scau.edu.cn
  • 基金资助:
    Supported by the Science and Technology Planning Project of Guangdong Province of China(2008B080701012);the Scientific Research Foundation for the Returned Overseas Chinese Scholars of Ministry of Education of China;the Leading Academic Discipline Program of Phase-3 of“Project-211”for South China Agricultural University(2009B010100001)

Biodegradation Kinetics of DDT in Soil under Different Environmental Conditions by Laccase Extract from White Rot Fungi

ZHAO Yuechun1, YI Xiaoyun2, LI Minghua1, LIU Lu1, MA Weijuan1   

  1. 1. Department of Applied Chemistry, College of Science, South China Agricultural University, Guangzhou 510642, China;
    2. College of Environmental Science and Engineering, South China University of Science&Technology, Guangzhou 510640, China
  • Received:2010-01-16 Revised:2010-03-29 Online:2010-06-28 Published:2010-06-28
  • Supported by:
    Supported by the Science and Technology Planning Project of Guangdong Province of China(2008B080701012);the Scientific Research Foundation for the Returned Overseas Chinese Scholars of Ministry of Education of China;the Leading Academic Discipline Program of Phase-3 of“Project-211”for South China Agricultural University(2009B010100001)

摘要: Biodegradation of 2,2-bis(p-chlorophenyl)-1,1,1-trichloroethane(DDT)in soil by laccase extract from white rot fungi under different experimental conditions was investigated.DDTs,which stands for the sum of p,p′-DDE,o,p′-DDT,p,p′-DDD and p,p′-DDT in soil was degraded efficiently,and the residue decreased rapidly during the first 15 days and then slowly during the period of 16-25 days.The biodegradation of DDTs in soil fitted the pseudo-first-order kinetics.For 5,10,15 and 25 days of incubation with laccase,the residue of DDTs in soil under different atmospheres was decreased by 20%-33%,34%-52%,41%-61%and 41%-69%respectively,under different flooding conditions that was decreased by 12%-17%,17%-30%,30%-45%and 35%-52%respectively, and for different soils that was decreased by 25%-34%,39%-53%,44%-58%and 47%-62%respectively.The half-life of DDTs in soil ranged from 15.07 to 32.95 days under O2,air or N2 atmospheres,23.07 to 40.71 days under different flooding conditions,and 18.78 to 28.88 days for different soils.Laccase is an efficient and safe agent for bioremediation of DDT-contaminated soil.

关键词: biodegradation, DDT, soil, laccase, atmosphere, flooding condition

Abstract: Biodegradation of 2,2-bis(p-chlorophenyl)-1,1,1-trichloroethane(DDT)in soil by laccase extract from white rot fungi under different experimental conditions was investigated.DDTs,which stands for the sum of p,p′-DDE,o,p′-DDT,p,p′-DDD and p,p′-DDT in soil was degraded efficiently,and the residue decreased rapidly during the first 15 days and then slowly during the period of 16-25 days.The biodegradation of DDTs in soil fitted the pseudo-first-order kinetics.For 5,10,15 and 25 days of incubation with laccase,the residue of DDTs in soil under different atmospheres was decreased by 20%-33%,34%-52%,41%-61%and 41%-69%respectively,under different flooding conditions that was decreased by 12%-17%,17%-30%,30%-45%and 35%-52%respectively, and for different soils that was decreased by 25%-34%,39%-53%,44%-58%and 47%-62%respectively.The half-life of DDTs in soil ranged from 15.07 to 32.95 days under O2,air or N2 atmospheres,23.07 to 40.71 days under different flooding conditions,and 18.78 to 28.88 days for different soils.Laccase is an efficient and safe agent for bioremediation of DDT-contaminated soil.

Key words: biodegradation, DDT, soil, laccase, atmosphere, flooding condition